We narrowed to 17,704 results for: SHE

1. pDNR-hSox2

   Plasmid

   #49389

   Purpose

   donor vector for cloning human Sox2 with 6xHN tag

   Depositor

   Randall McKinnon

   Insert

   Sox2 (SOX2 Human)
   

   Use

   Cre/Lox; Donor vector

   Tags

   6xHN (6 repeating His-Asn subunits)

   Mutation

   TGA (stop) to CCA (Pro) at nt 1027 with respect t…

   Promoter

   none

   Available Since

   Nov. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

2. Mouse Alpha2 delta-2 mutMIDAS HA pMT2

   Plasmid

   #58733

   Purpose

   expression of HA tagged mouse Alpha2 delta-2 with MIDAS site mutations in mammalian cells

   Depositor

   Annette Dolphin

   Insert

   alpha2 delta-2 (Cacna2d2 Mouse)
   

   Expression

   Mammalian

   Mutation

   MIDAS site mutations (D300VSGS to AVAGA)

   Promoter

   SV40

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSA109

   Plasmid

   #59783

   Purpose

   drives ZIF-1 protein in C. elegans intestinal cells

   Depositor

   Jeremy Nance

   Article

   Armenti et al Development. 2014 Dec;141(23):4640-7. do

   Inserts

   elt-2 promoter (elt-2 Nematode)
   zif-1 coding sequence (zif-1 Nematode)
   unc-54 3' UTR
   

   Available Since

   Nov. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-fishRab8A[T22N]

   Plasmid

   #24906

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   fishRab8A
   

   Use

   Gateway entry vector

   Mutation

   Changed Threonine 22 to Asparagine

   Available Since

   July 7, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pJK571

   Plasmid

   #75379

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Gene synthesis

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes glycine-209 to aspartate and synthetic ge…

   Promoter

   n/a

   Available Since

   Aug. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBACgus N-His/GST/LRRK2 1867-2176

   Plasmid

   #40406

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   GST-Tev and HisTev

   Mutation

   WT aa1867-2176

   Promoter

   polH

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

   Plasmid

   #50888

   Purpose

   Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. GRM3 (3SM9)

   Plasmid

   #32870

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   GRM3 (GRM3 Human)
   

   Tags

   His tag with TEV cleavage and Honeybee melittin S…

   Expression

   Bacterial

   Available Since

   Jan. 13, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. Hs.KRAS4b K104A

   Plasmid

   #83152

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K104A mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   K104A

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Hs.KRAS4b K104Q

    Plasmid

    #83153

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), K104Q mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    K104Q

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPS1358

    Plasmid

    #8918

    Depositor

    Pamela Silver

    Article

    Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

    Insert

    HRP1 (HRP1 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. ABL2

    Plasmid

    #39178

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ABL2 (ABL2 Human)
    

    Use

    Baculovirus expression

    Tags

    His Tev

    Mutation

    Truncated (see comments)

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. CUL5

    Plasmid

    #38975

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CUL5 (CUL5 Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Mouse Cul5 (GI:239051067) with two point mutation…

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. POT2-CYC1p-ycrtE-ADH1t

    Plasmid

    #65354

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    CYC1p-ycrtE-ADH1t
    

    Use

    Unspecified

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. POT4-CYC1p-ycrtI-ADH1t

    Plasmid

    #65357

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    CYC1p-ycrtI-ADH1t
    

    Use

    Unspecified

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6P-1-GST-CD-ATLm(Cbx7)

    Plasmid

    #82528

    Purpose

    Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7 amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 7 (CBX7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-84 with mutation of RKR70-72, K75, …

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hs.KRAS4b G12F

    Plasmid

    #83149

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12F mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12F

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hs.KRAS4b S181E

    Plasmid

    #83138

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S181E mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    S181E

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hs.KRAS4b S181D

    Plasmid

    #83137

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S181D mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    S181D

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Hs.KRAS4b S181A

    Plasmid

    #83136

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S181A mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    S181A

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pENTR-fishRab8A[Q67L]

    Plasmid

    #24905

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    fishRab8A
    

    Use

    Gateway entry vector

    Mutation

    Changed Glutamine 67 to Leucine

    Available Since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

22. POT4-TEF2p-ycrtI-ADH1t

    Plasmid

    #65356

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TEF2p-ycrtI-ADH1t
    

    Use

    Unspecified

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDNR-hAkt1

    Plasmid

    #49387

    Purpose

    donor vector for cloning human Akt1 with 6xHN tag

    Depositor

    Randall McKinnon

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Cre/Lox; Donor vector

    Tags

    6xHN (6 repeating His-Asn subunits)

    Mutation

    tga to cca at nt 1725 with respect to GenBank ID …

    Promoter

    none

    Available Since

    Dec. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

24. GUCY1A3

    Plasmid

    #39120

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GUCY1A3 (GUCY1A1 Human)
    

    Tags

    His6-Thioredoxin-TEV

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

25. GUCY1B3

    Plasmid

    #39035

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GUCY1B3 (GUCY1B1 Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

26. HSD17B14

    Plasmid

    #39160

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD17B14 (HSD17B14 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

27. BBOX1

    Plasmid

    #39176

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BBOX1 (BBOX1 Human)
    

    Use

    Baculovirus expression

    Tags

    His Tev

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pBS Caronte (CT#88)

    Plasmid

    #13859

    Depositor

    Cliff Tabin

    Article

    Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

    Insert

    Caronte (CER1 Chicken)
    

    Expression

    Bacterial

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

29. pTOPO-rRNA-5‘ETS 347 to 631

    Plasmid

    #23994

    Depositor

    Susan Baserga

    Article

    Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

    Insert

    rRNA 5'ETS ntds 347 to 631
    

    Use

    Bacterial cloning - topo

    Mutation

    None

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pBACgus4x/cdc37/LRRK2 1867-2186_C2024S/C2025S

    Plasmid

    #40366

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    HisTev

    Mutation

    aa1867-2186, C2024S/C2025S

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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