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Plasmid

#227583

Purpose

Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Insert

guide RNA scaffold

Use

Cloning vector

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pPP085

Plasmid

#158795

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLG011 (DRT type 3)

Plasmid

#157889

Purpose

Expresses the DRT type 3 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 3 (RT + RT)

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLG009 (DRT type 1)

Plasmid

#157887

Purpose

Expresses the DRT type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 1 (RT-nitrilase)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Pthl_fU

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLG007 (Ec86 retron)

Plasmid

#157885

Purpose

Expresses the Ec86 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec86 retron

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLG005 (Retron-TIR)

Plasmid

#157883

Purpose

Expresses the retron-TIR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Retron-TIR

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Expression

Bacterial

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSK9065

Plasmid

#84345

Purpose

Tightly controlled expression of mRFP fusion proteins in S. aureus

Depositor

Neville Firth

Article

Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

Type

Empty backbone

Tags

mRFPmars

Expression

Bacterial

Promoter

Pxyl/tet

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0

Plasmid

#153291

Purpose

Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

EFS

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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PrecA-CFP--PrecA-YFP

Plasmid

#228097

Purpose

recA promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

Depositor

Mary Dunlop

Article

Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

Insert

CFP & YFP

Expression

Bacterial

Promoter

recA & recA

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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mito-mScarlet

Plasmid

#141153

Purpose

Fluorescent labeling of the mitochondrial matrix in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

COX4 (COX4 Budding Yeast)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Tp-SceI-pheS

Plasmid

#111608

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5R MCS+

Plasmid

#211354

Purpose

empty vector control

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCB-CRISPRi

Plasmid

#221136

Purpose

Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

cbu1169 promoter

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAM5057

Plasmid

#120085

Purpose

Broad host range plasmid. Theophylline inducible expression of YFP in various cyanobacteria.

Depositor

Susan Golden

Article

Ma et al Appl Environ Microbiol. 2014 Nov;80(21):

Insert

RSF1010 Y25F aadA PconII theophylline riboswitch F with myc-yfp reporter

Use

Other

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLG012 (DRT type 4)

Plasmid

#157890

Purpose

Expresses the DRT type 4 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 4

Expression

Bacterial

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLG013 (DRT type 5)

Plasmid

#157891

Purpose

Expresses the DRT type 5 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 5

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pC0041-RanCas13b crRNA backbone

Plasmid

#103852

Purpose

For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

U6-RanCas13b DR-BbsI-BbsI-polyT

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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