We narrowed to 6,180 results for: cas9 expression plasmid

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Suntag_PRDM9_FWA_g4

Plasmid

#249560

Purpose

dCas9_Suntag_PRDM9 targeting FWA promoter

Depositor

Jake Harris

Article

Binenbaum et al Nat Commun. 2025 Oct 31;16(1):9587. doi:

Insert

PRDM9 (Prdm9 Mouse)

Use

Synthetic Biology

Tags

HA and sfGFP

Expression

Plant

Promoter

UBQ10

Available Since

Feb. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti_PE7-2A-BSD

Plasmid

#234073

Purpose

For lentiviral expression of PE7 with blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PE7

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_PEmax-2A-MLH1dn-2A-BSD

Plasmid

#234072

Purpose

For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PEmax-2A-MLH1dn

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-CTCF-prom

Plasmid

#195104

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF promoter

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA1-CTCF-prom

Plasmid

#195103

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF promoter

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-DNMT3a-DNMT3l

Plasmid

#154140

Purpose

Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP

Tags

HA and sfGFP

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPR_RAF1_94

Plasmid

#111826

Purpose

Knockout Raf1

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting RAF1 94-114 (RAF1 Human)

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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BPK1520_puroR

Plasmid

#173901

Purpose

SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

Depositor

Ronald Cohn

Article

Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV/U6

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLP857_α-syn:oScarlet KI donor

Plasmid

#239403

Purpose

AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

oScarlet

Use

AAV and CRISPR

Tags

oScarlet

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM07

Plasmid

#233479

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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AAV8-cPE-C

Plasmid

#183539

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase

Use

AAV

Expression

Mammalian

Promoter

U1A

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMTKM05

Plasmid

#233477

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM01

Plasmid

#233473

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Nat selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM03

Plasmid

#233475

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and G418 selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_PSMD1_sgRNA_1

Plasmid

#155107

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene)

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

PSMD1_sgRNA_1 (PSMD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-KO-4-EF1a-LibVec

Plasmid

#239600

Purpose

expresses guide#4 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-KO-3-EF1a-LibVec

Plasmid

#239599

Purpose

expresses guide#3 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-KO-2-EF1a-LibVec

Plasmid

#239598

Purpose

expresses guide#2 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-KO-1-EF1a-LibVec

Plasmid

#239597

Purpose

expresses guide#1 to knock-out mouse Plxnb2, via CRISPR-Cas9

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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