We narrowed to 146,921 results for: ins;

1. pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321)

   Plasmid

   #174640

   Purpose

   Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-GFP-GCN4-ppKinVIb(861-1321)
   

   Use

   Tags

   SSPB(micro)-GFP

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2PH-PLCd-APEX2-P2A-mKate2

   Plasmid

   #71284

   Purpose

   Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   2PH-PLCd-APEX2-P2A-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

   Plasmid

   #60418

   Purpose

   Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   mTiam1-tgRFPt-SSPB R73Q
   

   Use

   Cre/Lox and Lentiviral

   Tags

   tgRFPt

   Expression

   Mammalian

   Mutation

   mTiam 64-437 // SSPB R73Q, Y11K and A15E

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATG-1929

   Plasmid

   #108712

   Purpose

   CBR2opt in pF4Ag expression vector with T7 and CMV promoter

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   T7, CMV

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST40-2XFL-Src-S4-KD

   Plasmid

   #140297

   Purpose

   Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   contains only the SH4 and KD regions

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB80-SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)

   Plasmid

   #174643

   Purpose

   Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(nano)-GFP-GCN4-ppKin14VIb(861-1321)
   

   Use

   Tags

   SSPB(nano)-GFP

   Expression

   Mammalian

   Mutation

   EGFP: Met1Del; ppKin14VIb(aa861-1321)

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-DIO hChR2(E123A)-EYFP

   Plasmid

   #35507

   Purpose

   AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa-hChR2(E123A)-mCherry

   Plasmid

   #35506

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO-FNTA-FLAG

   Plasmid

   #86858

   Purpose

   To express FNTA with a FLAG tag at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   FNTA (FNTA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The sequence of the insert is isoform 2, which di…

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-hChR2(E123A)-EYFP

    Plasmid

    #35505

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. FAK2_HUMAN_D0

    Plasmid

    #79747

    Purpose

    This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    FAK2 (PTK2B Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCsy_complex

    Plasmid

    #89232

    Purpose

    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Inserts

    Csy1
    Csy2
    Csy3
    Csy4
    

    Use

    Tags

    6xHis-TEV protease

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

    Plasmid

    #170175

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-ST-P2A-NLS-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid

    #160140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(nucleoplasmin)-3xHA-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2-CreLite

    Plasmid

    #131783

    Purpose

    Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC, PIF6CreN, and mTagBFP2
    

    Use

    Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Ubi

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AICSDP-55:DMD-mEGFP

    Plasmid

    #159744

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS-CG-WT-Cer-VWF

    Plasmid

    #124827

    Purpose

    Expresses multimeric human VWF-ECFP fusion protein. The cyan fluorescence protein coding region is inserted between the VWF-D'D3 and A1 domains.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF with cyan fluorescent protein fusion between D'D3 and A1 domains (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET32a-hBTC

    Plasmid

    #199230

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Betacellulin (BTC Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-mCherry-C1-iChR2++

    Plasmid

    #98172

    Purpose

    artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iChR2++ gene
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    A59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid

    #208964

    Purpose

    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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