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We narrowed to 14,572 results for: pET

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pET28-sfGFP-DogTag Loop B

Plasmid

#171780

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop B

Use

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-Med25-VBD-6His

Plasmid

#64773

Purpose

Bacterial expression of His-tagged Med25-VBD

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25-VBD (MED25 Human)

Use

Tags

Expression

Bacterial

Mutation

insert is 394-543 only

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pET-17b_Unc104-KIF5B-HALO

Plasmid

#169996

Purpose

Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

Depositor

Ron Vale

Article

Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

Insert

Kinesin-like protein Unc-104 fused with KIF5B

Use

Tags

HALO-tag, 6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET-SUMO mCER-Prx2

Plasmid

#158226

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-dspB(E184Q)-6xHis

Plasmid

#175802

Purpose

Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-H12TEV-hEIF5B-1~1220(FL)

Plasmid

#208636

Purpose

for E. coli expression of human eIF5B (ENST00000289371)

Depositor

Quanfu Ma

Insert

human eIF5B (ENST00000289371) (EIF5B Human)

Use

Tags

6xhis

Expression

Bacterial

Mutation

Contain SNP rs1376443, protein sequence not chang…

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG 3.2

Plasmid

#193803

Purpose

Protein production of ChemoG3.2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG 3.2

Use

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET21b GFP-Rab10 Q68LHis

Plasmid

#186015

Purpose

GFP-Rab10 Q68LHis

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

GFP-Rab10 Q68LHis (RAB10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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PET-B-HypaSpCas9-NLS-6xHis

Plasmid

#207386

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A, amino acids 1005-1013…

Promoter

T7

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+36)-DL4

Plasmid

#199168

Purpose

used to express a supercharged GFP variant that forms inclusion bodies in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)-DL4

Use

Tags

MGHHHHHHGGA

Expression

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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