We narrowed to 10,617 results for: t7

1. pTRCHisB-TRF2_M

   Plasmid

   #50490

   Purpose

   E. Coli N-term HexHis Tagged Protein Expression Vector

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF2 Myb (TERF2 Human)
   

   Tags

   6x His, T7 tag, and TEV protease site

   Expression

   Bacterial

   Mutation

   Contains amino acids 438-500

   Promoter

   pTrc

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b_Qcat9

   Plasmid

   #45137

   Depositor

   Christopher Petzold

   Article

   Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

   Insert

   E-coli-pathways-QconCAT9
   

   Tags

   6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET29b_Qcat10

   Plasmid

   #45138

   Depositor

   Christopher Petzold

   Article

   Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

   Insert

   E-coli-pathways-QconCAT10
   

   Tags

   6XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTEV5_ RpMatB_4

   Plasmid

   #78208

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - weak binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A R286C T303A Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A R286C T303A Q457C K488A

   Promoter

   T7

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTEV5_ RpMatB_3

   Plasmid

   #78207

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - very weak binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A T167A R286C Q457C K488A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A T167A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29b_Qcat3

   Plasmid

   #45131

   Depositor

   Christopher Petzold

   Article

   Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

   Insert

   E-coli-pathways-QconCAT3
   

   Tags

   5XHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-GST-Q11

   Plasmid

   #185790

   Purpose

   Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   GST-Q11
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-TALE-mCherry-15b

   Plasmid

   #138287

   Purpose

   Express tTALE-mCherry

   Depositor

   Kyubong Jo

   Article

   Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

   Insert

   7-mer tTALE-mCherry
   

   Use

   TALEN

   Tags

   6x His-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-BirA-eCPX-AP(K10)

   Plasmid

   #121908

   Purpose

   Expresses BirA and eCPX-AP(K10)

   Depositor

   Per Svenningsen

   Article

   Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

   Inserts

   BirA (birA Synthetic)
   enhanced Circularly Permuted Outer Membrane Protein X
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 10 in the AP to alanine

   Promoter

   T7 and pBAD promoter

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. D. vulgaris Cas7/pHMGWA

    Plasmid

    #81184

    Purpose

    Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas7
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sc NybbR eRF3∆165N (WT)

    Plasmid

    #175419

    Purpose

    Encodes His-MBP-TEV-ybbR-WT eRF3∆165N

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF3 (SUP35 Budding Yeast)
    

    Tags

    His-MBP-TEV and ybbR

    Expression

    Bacterial

    Mutation

    ∆165N

    Promoter

    T7

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b/EYFP-GgVcl 1-851

    Plasmid

    #46264

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-851 (VCL Chicken)
    

    Tags

    EYFP and His

    Expression

    Bacterial

    Mutation

    aa 1-851 (aka Vh) head domain

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 CTTN

    Plasmid

    #67100

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTTN (CTTN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-BCA-Pep-H

    Plasmid

    #185782

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Pep-H
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET151-mUCH37 (ISF2)

    Plasmid

    #61928

    Purpose

    Expression of mUCH37 (ISF2) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    UCH37 (M.musculus) (Uchl5 Mouse)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDC146

    Plasmid

    #169366

    Purpose

    Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96

    Depositor

    Axel Brunger

    Article

    Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45

    Insert

    Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    

    Tags

    6 his tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29b_Qcat5

    Plasmid

    #45133

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT5
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET29b_Qcat2

    Plasmid

    #45130

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT2
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b_Qcat6

    Plasmid

    #45134

    Depositor

    Christopher Petzold

    Article

    Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

    Insert

    E-coli-pathways-QconCAT6
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Pediculus humanus corporis PINK1 (143 - 575)

    Plasmid

    #110751

    Purpose

    Bacterial expression of PhPINK1 (crystallography)

    Depositor

    David Komander

    Article

    Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

    Insert

    Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
    

    Tags

    N-His6-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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