We narrowed to 7,668 results for: CCH

1. BAS289 C terminal E2C G418R

   Plasmid

   #74084

   Purpose

   S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, G418 resistance

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   E2Crimson
   

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pU6Rosa-CAG-Gal4-Rad18UBD

   Plasmid

   #119012

   Purpose

   Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA3

   Plasmid

   #46790

   Purpose

   To express ATPase inactive Fun30 in budding yeast

   Depositor

   Patrick Varga-Weisz

   Article

   Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

   Insert

   FUN30 (FUN30 Budding Yeast)
   

   Tags

   V5

   Expression

   Yeast

   Mutation

   ATP-binding site mutated replaced lysin 603 by ar…

   Promoter

   PGAL1

   Available Since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pDONR221-RAP1

   Plasmid

   #84525

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Mutation

   no stop codon

   Promoter

   none

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNM3746

   Plasmid

   #154109

   Purpose

   loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Inserts

   LoxP-SEC-LoxP
   FRT::rpl-28p::GFP::His-58::FRT3
   mex-5p::FLP D5::sl2::mNG::glh2 3'
   

   Use

   Cre/Lox; Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2799

   Plasmid

   #63658

   Purpose

   pCfB2799 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB2992

   Plasmid

   #63642

   Purpose

   pCfB2992 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   none

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP84

   Plasmid

   #83542

   Purpose

   Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   SSA1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   SSA1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH734-CEN-minHIS3

   Plasmid

   #38227

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   HIS3 (HIS3 Budding Yeast, Synthetic)
   

   Tags

   HA

   Expression

   Yeast

   Mutation

   Every codon of the coding sequence has been repla…

   Promoter

   TDH3

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTH737-CEN-maxHIS3

    Plasmid

    #38229

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Budding Yeast, Synthetic)
    

    Tags

    HA

    Expression

    Yeast

    Mutation

    Every codon of the coding sequence has been repla…

    Promoter

    TDH3 (=GPD)

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-RLuc8C-FHA1-NES

    Plasmid

    #138210

    Purpose

    Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    RLuc8C-FHA1-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. A102 ΔAmpH Δ2x+ve

    Plasmid

    #129484

    Purpose

    Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

    Depositor

    Blanche Schwappach

    Article

    Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

    Insert

    ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKV005

    Plasmid

    #64763

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKP4010

    Plasmid

    #113336

    Purpose

    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Inserts

    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    

    Tags

    3X-FLAG/8X-Gly linker

    Expression

    Yeast

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BAS291 C terminal mKO2 kanR

    Plasmid

    #74086

    Purpose

    S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    mKO2
    

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

    Plasmid

    #58830

    Purpose

    Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

    Insert

    2 x Osh2 PH domain (OSH2 Budding Yeast)
    

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    changed Lysine 307 to Glutamate and Arginine 309 …

    Promoter

    PHO5

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGS1011

    Plasmid

    #63783

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker

    Depositor

    Bjoern Sander, Monika Golas

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Strep-tag II followed by TRP1 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Strep-tag II

    Promoter

    none

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. BAS290 C terminal E2C natR

    Plasmid

    #74085

    Purpose

    S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistance

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    E2Crimson
    

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pRS313-PRP8

    Plasmid

    #90964

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    native

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 720-2µ-maxHIS3

    Plasmid

    #40612

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Budding Yeast, Synthetic)
    

    Tags

    HA

    Expression

    Yeast

    Mutation

    Every codon of the coding sequence has been repla…

    Promoter

    TDH3

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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