We narrowed to 10,828 results for: 158
-
Plasmid#220240PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
Tags6x His, MBPExpressionInsectMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterPolyhedrinAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt1
Plasmid#220239PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
Tags6x His, MBPExpressionInsectMutationR27A, R31A, F165APromoterPolyhedrinAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT360 AAV-Rapamycin Inducible-noActivator Domain -Citrine
Plasmid#202058PurposeInducible AAV with reporter gene and cloning site to add activator domainsDepositorInsertAAV-Rapamycin Inducible-noActivator Domain -Citrine
UseAAV; Aav with reporterExpressionMammalianMutationITRs intactAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT312 AAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator
Plasmid#202061PurposeInducible AAV activatorDepositorInsertAAV-Rapamycin Inducible-NFZ-Citrine with sNRP-1 terminator
UseAAV; Aav with reporterExpressionMammalianMutationITRs intactAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-ePhi29(+exo) (LM2995)
Plasmid#208966PurposeUnfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.DepositorInsertePhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…PromoterCMV and T7Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PDGFR-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-tTA
Plasmid#210502Purposeexpresses LAUNCHERtvmv component in mammalian cellsDepositorInsertPDGFR-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-tTA
TagsMycExpressionMammalianPromoterCMVAvailable SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)
Plasmid#208973PurposeRecruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUt-mNF-GFP
Plasmid#199220PurposeLPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInserthTetR::P2A::EGFP
TagsEGFPExpressionMammalianPromoterCMV D2ir promoterAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Gata6-3xT7
Plasmid#200889PurposeInducible lentiviral expression of Gata6DepositorInsertGata6 (Gata6 Mouse)
UseLentiviral; Doxycycline inducibleTags3xT7ExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSI-218
Plasmid#197139PurposeAll-in-vector of SpCas9 gRNA cloning sites (BsmBI) and CMVp-Target-AID expression unitDepositorTypeEmpty backboneUseCRISPRTagsSpCas9-PmCDA1-UGIExpressionMammalianMutationSpCas9(D10A)Available SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD
Plasmid#148514PurposeBacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83DepositorInsertDmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD
Plasmid#148515PurposeBacterial Expression of DmThor_50-63-DmeIF4Ga_631-675DepositorInsertDmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-CG32016-E_T
Plasmid#147590PurposeInsect Expression of DmThor-CG32016-EDepositorInsertDmThor-CG32016-E (Thor Fly)
ExpressionInsectMutationDmThor two silent mutations compared to the seque…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-DmCup_417-1133_T
Plasmid#147591PurposeInsect Expression of DmThor-DmCup_417-1133DepositorInsertDmThor-DmCup_417-1133 (Thor Fly)
ExpressionInsectMutationThor contains two silent mutations A246C and T321…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmThor-DmeIF4Ga_687-1666_T
Plasmid#147592PurposeInsect Expression of DmThor-DmeIF4Ga_687-1666DepositorInsertDmThor-DmeIF4Ga_687-1666 (Thor Fly)
ExpressionInsectMutationDmThor two silent mutations A246C and T321C compa…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-KIR2DS5-COMP5AP-AviTag-9xHis
Plasmid#157238PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hPEN2 20A
Plasmid#183639PurposeLentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1.DepositorInsertPEN2 (PSENEN Human)
UseLentiviralTagsHAMutationresidues 27, 28, 30, 31, 34, 38, 42, 43, 57, 58, …PromoterCMVAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF532
Plasmid#88740PurposeDonor Vector containing ZNF532 transcription factor, part of the Human TFome CollectionDepositorInsertZNF532 (ZNF532 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF77
Plasmid#88744PurposeDonor Vector containing ZNF77 transcription factor, part of the Human TFome CollectionDepositorInsertZNF77 (ZNF77 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF672
Plasmid#88753PurposeDonor Vector containing ZNF672 transcription factor, part of the Human TFome CollectionDepositorInsertZNF672 (ZNF672 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF678
Plasmid#88761PurposeDonor Vector containing ZNF678 transcription factor, part of the Human TFome CollectionDepositorInsertZNF678 (ZNF678 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF688_isoform1
Plasmid#88779PurposeDonor Vector containing ZNF688 transcription factor, part of the Human TFome CollectionDepositorInsertZNF688 (ZNF688 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF688_isoform2
Plasmid#88783PurposeDonor Vector containing ZNF688 transcription factor, part of the Human TFome CollectionDepositorInsertZNF688 (ZNF688 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF688_isoform3
Plasmid#88787PurposeDonor Vector containing ZNF688 transcription factor, part of the Human TFome CollectionDepositorInsertZNF688 (ZNF688 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF30
Plasmid#88681PurposeDonor Vector containing ZNF30 transcription factor, part of the Human TFome CollectionDepositorInsertZNF30 (ZNF30 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF705D
Plasmid#88684PurposeDonor Vector containing ZNF705D transcription factor, part of the Human TFome CollectionDepositorInsertZNF705D (ZNF705D Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF669
Plasmid#88688PurposeDonor Vector containing ZNF669 transcription factor, part of the Human TFome CollectionDepositorInsertZNF669 (ZNF669 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF420
Plasmid#88703PurposeDonor Vector containing ZNF420 transcription factor, part of the Human TFome CollectionDepositorInsertZNF420 (ZNF420 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF792_isoform1
Plasmid#88704PurposeDonor Vector containing ZNF792 transcription factor, part of the Human TFome CollectionDepositorInsertZNF792 (ZNF75A Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF829_isoform2
Plasmid#88707PurposeDonor Vector containing ZNF829 transcription factor, part of the Human TFome CollectionDepositorInsertZNF829 (ZNF829 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only