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AT5G62710 X045_pECIA2

Plasmid

#115130

Purpose

Bait vector AT5G62710 X045_pECIA2 should be used with prey vector AT5G62710 X045_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G62710 (AT5G62710 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168 delta MIU1/MIU2

Plasmid

#133981

Purpose

mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU1 and MIU2

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pZE21/UBP1/ClpS

Plasmid

#98566

Purpose

Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

Depositor

George Church

Article

Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

Inserts

Truncated ubiquitin cleavase, codon optimized
ATP-dependent Clp protease adapter protein

Expression

Bacterial

Mutation

In a synthetic operon downstream of UBP1 and Remo…

Promoter

Same as UBP1 (operon) and Tet promoter (aTc induc…

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_WNT2_WT

Plasmid

#81775

Purpose

Gateway Donor vector containing WNT2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WNT2 (WNT2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AT1G35710 X075_pECIA2

Plasmid

#115098

Purpose

Bait vector AT1G35710 X075_pECIA2 should be used with prey vector AT1G35710 X075_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G35710 (AT1G35710 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G56140 X032_pECIA2

Plasmid

#115062

Purpose

Bait vector AT1G56140 X032_pECIA2 should be used with prey vector AT1G56140 X032_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G56140 (AT1G56140 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G06840 X062_pECIA2

Plasmid

#115052

Purpose

Bait vector AT1G06840 X062_pECIA2 should be used with prey vector AT1G06840 X062_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G06840 (AT1G06840 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT2G19230 M11_pECIA2

Plasmid

#114949

Purpose

Bait vector AT2G19230 M11_pECIA2 should be used with prey vector AT2G19230 M11_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G19230 (AT2G19230 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G29730 X066_pECIA14

Plasmid

#114855

Purpose

Prey vector AT1G29730 X066_pECIA14 should be used with bait vector AT1G29730 X066_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G29730 (AT1G29730 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT3G02880 J3_pECIA14

Plasmid

#114790

Purpose

Prey vector AT3G02880 J3_pECIA14 should be used with bait vector AT3G02880 J3_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G02880 (AT3G02880 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G51890 M5_pECIA14

Plasmid

#114744

Purpose

Prey vector AT1G51890 M5_pECIA14 should be used with bait vector AT1G51890 M5_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G51890 (AT1G51890 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

Plasmid

#106190

Purpose

Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184V

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

Promoter

CAG

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 SARS-CoV-2 S-frag5_nostop

Plasmid

#153177

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag5

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pS6-TGFB2-STREP

Plasmid

#128503

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Tags

STREP (2X) tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV2 TEV-N

Plasmid

#154424

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

N (N SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available Since

Sept. 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pPB-cT3G-cERP2-FOSB

Plasmid

#192901

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of FOSB

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOSB (FOSB Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_SV2C_WT

Plasmid

#81924

Purpose

Gateway Donor vector containing SV2C, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SV2C (SV2C Human)

Use

Gateway entry vector

Mutation

F346S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v3 (-cyt)

Plasmid

#137813

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v3 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region, N280D in the V3 region

Promoter

PGK

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

Plasmid

#170254

Purpose

Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

STAT3 (STAT3 Human)

Use

Lentiviral

Mutation

A662C, N664C, V667L

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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ACTB-(A)3x

Plasmid

#112057

Purpose

ACTB mRNA tagged with 3 copies of Riboglow (variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

ACTB (ACTB Human)

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

3 copies of Riboglow tag (variant A) after stop c…

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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