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Plasmid#232997PurposeGalactose iduced expression of Gcn4 9acidAHA in yeastDepositorInsertGcn4 9acidA
UseTags3xHA and TEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 9acidAGFP
Plasmid#233009PurposeGalactose iduced expression of Gcn4 9acidAGFP in yeastDepositorInsertGcn4 9acidA
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationE109A, E111A, E114A, D115A, E119A, D125A, D127A, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+GFP
Plasmid#233002PurposeGalactose iduced expression of Gcn4 Aro3+GFP in yeastDepositorInsertGcn4 Aro3+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 ILVtoED WGFP
Plasmid#233007PurposeGalactose iduced expression of Gcn4 LVtoED W+GFPin yeastDepositorInsertGcn4 ILVtoED W+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationN126W, I128E, V130E, V135E, L137D, I142DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 solvvol W
Plasmid#231878PurposeBacterial expression of N-terminally 6His tagged Gcn4 solvvol WDepositorInsertGcn4 solvvol W
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 solvvol
Plasmid#231877PurposeBacterial expression of N-terminally 6His tagged Gcn4 solvvolDepositorInsertGcn4 solvvol
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 SATtoG KtoR
Plasmid#231861PurposeBacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoRDepositorInsertGcn4 SATtoG KtoR
UseTags6xHis, TEV cleavage siteExpressionBacterialMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
DMTF1
Plasmid#36895PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDMTF1 (DMTF1 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains Myb-like 1 domain, residues 220-274PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
L284F Cus2p
Plasmid#73059PurposeS. cerevisiae Cus2p gene with L284F MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationLeucine 284 to PhenylalaninePromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
D282N Cus2p
Plasmid#73060PurposeS. cerevisiae Cus2p gene with D282N MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationAsp 282 to AsnPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
KRAS-P34R
Plasmid#158527PurposeExpresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTet-On-TAP
Plasmid#59017PurposeConstruct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.DepositorTypeEmpty backboneUseToxoplasma expressionTagsCalmodulin Binding Peptide fused with a TEV Cleav…ExpressionMutationPromoterRPS13 with Tet Operators InsertedAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (1-575)
Plasmid#1318DepositorInsertHsp104 (1-575) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationaa1-575 of Hsp104PromoterAvailable sinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
dFP-mini
Plasmid#120543PurposeExpresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.DepositorInsertde novo fluorescence-activating protein mini (dFP-mini)
UseTags6xHis - TEV cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
PsaA
Plasmid#159997PurposeExpresses Streptococcus pneumoniae PsaA (19-309) in bacterial cellsDepositorInsertPsaA (19-309)
UseTagsHis6-tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Spy0469
Plasmid#159996PurposeExpresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cellsDepositorInsertSpy0469 (24-389)
UseTagsHis6-tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-ICL1
Plasmid#105822PurposeExpression of Mycobacterium tuberculosis icl1 recombinant proteinDepositorInserticl1
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-CHAD
Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
FnR9-10
Plasmid#182567PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only