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pOPINK-Sharpin NZF (347-376)

Plasmid

#235651

Purpose

Bacterial expression of human Sharpin NZF (347-376)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

Sharpin NZF (SHARPIN Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 347-376 only

Promoter

T7

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-i53-bpA-EF1BFP

Plasmid

#111145

Purpose

Expression of i53 and a BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

i53 (mutL Synthetic)
BFP (BFP Synthetic)

Expression

Mammalian

Mutation

human Ubiquitin, contains 8 point mutations

Promoter

CAG promoter and EF1

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

Plasmid

#191145

Purpose

Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

3UTR(Bdnf)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 (N)ZF1-3 (1-173)

Plasmid

#235646

Purpose

Bacterial expression of human CAPN15 (N)ZF1-3 (1-173)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 (N)ZF1-3 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 1-173 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 NZF2 (34-72)

Plasmid

#235645

Purpose

Bacterial expression of human CAPN15 NZF2 (34-72)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 NZF2 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 34-72 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 NZF1 (1-42)

Plasmid

#235644

Purpose

Bacterial expression of human CAPN15 NZF1 (1-42)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 NZF1 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 1-42 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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VirTREX2-HLDel-2_NbPDS

Plasmid

#231145

Purpose

T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

TREX2 and mobile gRNA targeting NbPDS

Expression

Plant

Promoter

PEBV sub-genomic

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDIRECT_25H

Plasmid

#91145

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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TRE-eGFP-F1C

Plasmid

#211457

Purpose

Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation blocking peptide (F1C)

Depositor

Linda DeGraffenried

Article

McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

Insert

Flotillin-1-palmitoylation blocking peptide (FLOT1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

None

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-eGFP-F1A

Plasmid

#211458

Purpose

Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation mutant control peptide (F1A)

Depositor

Linda DeGraffenried

Article

McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

Insert

Flotillin-1-palmitoylation mutant control peptide (FLOT1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

C34A

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINK-NPL4 NZF (574-608)

Plasmid

#235653

Purpose

Bacterial expression of human NPL4 NZF (574-608)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

NPL4 NZF (NPLOC4 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 574-608 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 ZF5 (411-444)

Plasmid

#235649

Purpose

Bacterial expression of human CAPN15 ZF5 (411-444)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 ZF5 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 411-444 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 ZF4 (337-369)

Plasmid

#235648

Purpose

Bacterial expression of human CAPN15 ZF4 (337-369)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 ZF4 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 337-369 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-CAPN15 ZF3 (141-173)

Plasmid

#235647

Purpose

Bacterial expression of human CAPN15 ZF3 (141-173)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

CAPN15 ZF3 (CAPN15 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 141-173 only

Promoter

T7

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pET28P_dCrk

Plasmid

#131145

Purpose

Expresses 6XHis::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Kv7.1/pcDNA3.1

Plasmid

#111452

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)

Tags

8xHis and EGFP

Expression

Mammalian

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-695)/pcDNA3.1

Plasmid

#111453

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

8xHis and EGFP

Expression

Mammalian

Mutation

Kv7.4 (1-695)

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCYC1pr-GNSI (LEU)

Plasmid

#111450

Purpose

GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

Depositor

Alexey Merz

Article

Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

Inserts

CYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator

Tags

mGFP5

Expression

Yeast

Mutation

M109I (Naturally occurring SNP) and The first 21 …

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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Kv7.4 (1-645, Δ368-523)/pcDNA3.1

Plasmid

#111456

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523), C643A

Available Since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-CAAX

Plasmid

#241145

Purpose

Expresses Anti-HA frankenbody-StayGold and HaloTag-tdMCP-CAAX to track mature and nascent SunTag-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-HA frankenbody
tdMCP - CAAX

Tags

(n2)oxStayGold(c4) E147D and HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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