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Plasmid#221183PurposecdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)DepositorInsertcdGreen2
UseTagsExpressionBacterialMutationPromoterPQ5 (cumate-inducible)Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pConRef-2H12
Plasmid#221182PurposecdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)DepositorInsertscdGreen
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
RhGC(BE)_pGEM
Plasmid#85469PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertrhodopsin-guanylyl cyclase
UseTagsExpressionMammalianMutationPromoterT7 PromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2H12Matry-blind
Plasmid#221181PurposecdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)DepositorInsertcdGreen-Matry(blind)
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Apr)
Plasmid#110085PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
hNedd4-2 CS
Plasmid#86041Purposefull length NeddL3 with C to S mutation with His Xpress tagDepositorInserthNeddL3 with C to S mutation in HECT domain (NEDD4 Human)
UseTagsHis and XpressExpressionMammalianMutationC942S mutation in HECT domainPromoterCMVAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTetR-BCD2-Ana GvpC
Plasmid#153298PurposeExpresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquaeDepositorInsertGas vesicle protein C
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
OccludinMut_Flag
Plasmid#86043PurposeFull length Occludin with mutation of PPPY domain at AA9-12 to PAAYDepositorInsertOccludinMut (OCLN Human)
UseTagsFLAGExpressionMammalianMutationPPPY domain at AA9-12 to PAAY; I52T and Q365PPromoterCMVAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)
Plasmid#225443PurposeGeminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.DepositorInsertBeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2H12ref
Plasmid#221188PurposecdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206)DepositorInsertscdGreen
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterNone (same as cdGreen) and PtetAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-RIPLET ΔPRY/SPRY
Plasmid#218516PurposeRIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1DepositorInsertRIPLET delta PRY/SPRY domain (RNF135 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-fsr1
Plasmid#179866PurposeEncodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiaeDepositorInsertFusarubin polyketide synthase
UseSynthetic BiologyTagsExpressionMutationCodon optimized for expression in Saccharomyces c…PromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3-2xStrep
Plasmid#170315PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22YU3 (TTHERM_00122270 Tetrahymena thermophila)
UseTagsStrep-Tag IIExpressionInsectMutationPromoterPolyhedrinAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3 del C3-2xStrep
Plasmid#170316PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 with a C-terminal truncation (C3-finger). Tagged with 2x Strep-tag II at the C-terminus.DepositorInsertQ22YU3 C-terminal truncation (Delta-C3)
UseTagsStrep-Tag IIExpressionInsectMutationDeletion of C-terminal C3-fingerPromoterpolyhedrinAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-CBD-hGLY
Plasmid#134596PurposeLow Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tagDepositorInserthuman glycine acyltransferase 2 (GLYATL2 )
UseTagschitin binding domainExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128347PurposepAAV encoding control gRNA for CRISPR gRNAs listed aboveDepositorInsertcontrol (negative)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pConRef-2H12.D11
Plasmid#221151PurposecdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)DepositorInsertscdGreen2
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen2)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBR15.2-2H12.D11opt-scarRef
Plasmid#221150PurposecdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)DepositorInsertscdGreen2.1
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen2.1)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT C-His Ana GvpC in pET28a
Plasmid#85732PurposeExpresses His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (his-tagged)
UseTagsHis-tagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-mOXT-hM3Dq-mCherry-WPRE
Plasmid#70717PurposeExpresses DREADDs Gq receptor under oxytocin promoter controlDepositorInserthM3Dq-mCherry
UseAAVTagsmCherryExpressionMutationPromotermouse oxytocinAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only