We narrowed to 33,487 results for: Eng
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Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
TagsHis and MBPExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK1043
Plasmid#8835DepositorInserttobacco etch virus protease, catalytic domain
TagsMBP and polyarginineExpressionBacterialMutationAutolysis-resistant S219V mutantAvailable SinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTS1017-Tier1-OTetO7-PCMVmin-SEAP
Plasmid#169586PurposeTier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
ExpressionMammalianPromoterTetO7-PCMVminAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-roGFP
Plasmid#202464PurposeExpresses redox sensitive GFP (roGFP) with T7 inductionDepositorInsertreduction-oxidation sensitive green fluorescent protein
Tags6X-HisExpressionBacterialPromoterT7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu5 (R)
Plasmid#98296PurposeEnhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
ExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)
Plasmid#83947PurposegRNA cassette-carrying vector with natMX markerDepositorInsertgRNA targeting ADE2 gene
ExpressionYeastAvailable SinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
negActinin-sstFRET-GR
Plasmid#83417PurposeFRET-based actinin tension sensor (negative control)DepositorInsertnegActinin-sstFRET
ExpressionMammalianPromoterCMVAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCKmatBC
Plasmid#138587PurposematBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, catDepositorInsertbicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
UseSynthetic BiologyExpressionBacterialMutationmatB contains native GUG start codonPromoterPlac promoterAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
NFkBr-IL1Ra
Plasmid#183065PurposeExpresses IL-1Ra downstream of a synthetic promoter activated by the NFkB signaling pathwayDepositorInsertinterleukin-1 receptor antagonist (Il1rn Mouse)
UseLentiviralAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK255
Plasmid#171155PurposeExpression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmidDepositorInsertI-SceI_sacB
ExpressionBacterialAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCN-mBFP
Plasmid#63847PurposeEncodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.DepositorInsertmBFP
ExpressionBacterialMutationPromoter and RBS was swapped. His-tag was removed…Available SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVH607-Tier1-PCREm4-Nluc
Plasmid#169594PurposeTier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).DepositorInsertcAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4Available SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p
Plasmid#202484PurposeExpresses sulfhydryl oxidase (Erv1p) constitutivelyDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
ExpressionYeastAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-VCAM1
Plasmid#202471PurposeExpresses the nanobody VCAM1 after phosphate depletionDepositorInsertnanobody VCAM1
Tags6X-HisExpressionBacterialPromoteryibDpAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS2374-Tier1-PhCMV-Gal4_VP16
Plasmid#169582PurposeTier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).DepositorInsertGAL4-transactivator
ExpressionMammalianPromoterPhCMVAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHYX137
Plasmid#202814PurposeSaccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.DepositorTypeEmpty backboneExpressionYeastPromoterScPCK1Available SinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only