We narrowed to 8,830 results for: Pol

1. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Use

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA

   Plasmid

   #89459

   Purpose

   constitutive expression of human CHD7 in mammalian cells

   Depositor

   Peter Scacheri

   Insert

   CHD7 (CHD7 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

   Plasmid

   #174028

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-eGFP
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

   Plasmid

   #174029

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-mScarlet
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-CAG-VARNAM A122D WPRE-bGH-polyA

   Plasmid

   #180486

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   VARNAM A122D
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense

   Plasmid

   #46835

   Purpose

   Express cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]

   Depositor

   Phil Sharp

   Article

   Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSF4 CMV intron renilla TRICK CTE polyA

   Plasmid

   #84443

   Purpose

   TRICK reporter mRNA

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   Renill-TRICK
   

   Use

   Luciferase; Frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-CMV

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

   Plasmid

   #208972

   Purpose

   Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29(+exo)-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-EGFPnls barcode-206-SV40 polyA

   Plasmid

   #190875

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFPnls-barcode206
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

    Plasmid

    #46836

    Purpose

    Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-CMV-Flag-PolB(K244A/T304I)-Puro

    Plasmid

    #177144

    Purpose

    Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSynI-mCherry-WPRE-hGH poly(A) (Alex_01)

    Plasmid

    #71650

    Purpose

    AAV expression of mCherry in human and mouse neurons

    Depositor

    Mirjana Maletić-Savatić

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynI

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)

    Plasmid

    #71651

    Purpose

    AAV expression of EGFP in human and mouse neurons

    Depositor

    Mirjana Maletić-Savatić

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA

    Plasmid

    #218870

    Purpose

    Mammalian expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2(no bind)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102G R168P

    Promoter

    CAG

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

    Plasmid

    #207135

    Purpose

    DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

    Plasmid

    #207136

    Purpose

    DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid

    #208970

    Purpose

    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-EGFPnls barcode-210-SV40 polyA

    Plasmid

    #190876

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFPnls-barcode210
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1C-no5UTR-Fluc-MtoL-V5-5BoxB-polyA-HhR_AC

    Plasmid

    #148409

    Purpose

    Insect Expression of 5BoxB-polyA-HhR

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    5BoxB-polyA-HhR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC

    Plasmid

    #148446

    Purpose

    Mammalian Expression of 6xbsMS2-PolyA-Malat

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    6xbsMS2-PolyA-Malat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge