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Addgene

We narrowed to 1,005 results for: Psp

Showing: 601 - 620 of 1005 results
  1. miniUAA6

    Plasmid
    #78485
    Purpose
    1559nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Insert
    CAN1-LUC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. miniUAA4

    Plasmid
    #78483
    Purpose
    1105nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Insert
    CAN1-LUC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. miniUAA2

    Plasmid
    #78481
    Purpose
    600nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Insert
    CAN1-LUC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. miniUAA3

    Plasmid
    #78482
    Purpose
    882nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Insert
    CAN1-LUC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pDB038a

    Plasmid
    #99211
    Purpose
    Accessory plasmid for PACE of PylRS variants
    Depositor
    Insert
    Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 29 and 184 of gIII
    Promoter
    Ppsp and PproK
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pDB038b

    Plasmid
    #99212
    Purpose
    Accessory plasmid for PACE of PylRS variants
    Depositor
    Insert
    Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 29, 83, and 184 of gIII
    Promoter
    Ppsp and PproK
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pDB007ns2a

    Plasmid
    #99214
    Purpose
    Accessory plasmid for PACE of PylRS variants; negative selection
    Depositor
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and Ptet
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pDB023f1

    Plasmid
    #99218
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    Insert
    Ppsp [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pDB026e

    Plasmid
    #109261
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants
    Depositor
    Insert
    Ppsp_pIII(P29*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codon at position 29 and 184 of gIII
    Promoter
    Ppsp and PproK
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pDB023k

    Plasmid
    #99219
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    Insert
    Ppsp [SD8] T7RNAP(S12*,S203*,S527*)
    Use
    Tags
    Expression
    Mutation
    amber codons at positions 12, 203, and 527 of T7 …
    Promoter
    Ppsp
    Available since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. pDB026g

    Plasmid
    #109263
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants
    Depositor
    Insert
    Ppsp_pIII(P29*, P83*, T177*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codon at position 29, 83, 177, and 184 of g…
    Promoter
    Ppsp and PproK
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. AAA

    Plasmid
    #78487
    Purpose
    2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Insert
    CAN1-LUC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CAN1 contains no stop codon
    Promoter
    Available since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. pC0051-W85X REPAIR targeting guide cloned into pC0043

    Plasmid
    #103867
    Purpose
    Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038
    Depositor
    Insert
    W85X targeting guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  14. pDB036d

    Plasmid
    #99216
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and PproA
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 601 - 620 of 1005 results