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pRhobin2-SEC61B-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239024

Purpose

Co-overexpression of Rhobin2-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin2-SEC61B (SEC61B Human, Synthetic)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PdTK-CAG-mCh-[uBglII]

Plasmid

#107280

Purpose

puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEcRNAP5

Plasmid

#128939

Purpose

pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

Depositor

Seth Darst

Article

Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

Inserts

RpoAppx234-241-(His)10 (rpoA Escherichia coli)
RpoB
RpoC
RpoZ

Tags

His

Expression

Bacterial

Mutation

Residues 234-241 are substituted for a PreScissio…

Promoter

T7

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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p3-HPRT1-S104R-PdTK-mCh

Plasmid

#107276

Purpose

HPRT1 c.312C>A Munich donor vector with unilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

HPRT genomic DNA (HPRT1 Human)
puro-deltaTK; CAG::mCherry

Mutation

c.306G>T, c.312C>A

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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p3-HPRT1-S104Rf-PdTK-mCh

Plasmid

#107277

Purpose

HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Inserts

HPRT genomic DNA (HPRT1 Human)
puro-deltaTK; CAG::mCherry

Mutation

c.306G>T, c.312C>A

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)-FLEx(loxP)-eYFP(nuclear)-2A-N2cG

Plasmid

#248664

Purpose

Helper virus for monosynaptic tracing; Expresses nuclear eYFP and Rabies Glycoprotein (G) of CVS-N2c strain in Cre-expressing cells under the CAG promoter.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Inserts

N2c(G)
nuc-eYFP

Use

AAV

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pRhobin9-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239021

Purpose

Co-overexpression of Rhobin9-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin9-H2B (H2BC21 Human, Synthetic)

Tags

TagBFP and mNeonGreen3K

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTOMM20-Rhobin2-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239022

Purpose

Co-overexpression of TOMM20-Rhobin2 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

TOMM20-Rhobin2 (TOMM20 Human, Synthetic)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTOMM20-Rhobin9-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239023

Purpose

Co-overexpression of TOMM20-Rhobin9 fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

TOMM20-Rhobin9 (TOMM20 Human, Synthetic)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRhobin9-SEC61B-IRES-NLS-mTagBFP-2xNLS

Plasmid

#239025

Purpose

Co-overexpression of Rhobin9-SEC61B fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

Depositor

William DeGrado, Bo Huang

Article

Chen et al bioRxiv 2025.06.24.661379

Insert

Rhobin9-SEC61B (SEC61B Human, Synthetic)

Tags

TagBFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

Plasmid

#202090

Purpose

Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2(R195G, C201A)

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

R195G and C201A abolish catalytic activity

Promoter

human Synapsin

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-SPN_ARR N52R (Shank3)

Plasmid

#175255

Purpose

SPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expression

Depositor

Johanna Ivaska

Article

Salomaa et al Curr Biol. 2021 Sep 29. pii: S0960-9822(

Insert

SPN-ARR fragment with N52R mutation from SHANK3

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pN1-PinkyCaMP

Plasmid

#232861

Purpose

A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

Depositor

Olivia Masseck

Article

Fink et al Nat Methods. 2026 Apr 24. doi: 10.1038/s

Insert

PinkyCaMP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pVax1-hVEGF165

Plasmid

#74466

Purpose

expression of hVEGF165 in mammalian cells

Depositor

Loree Heller

Article

Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

Insert

vascular endothelial growth factor 165 (VEGFA Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG-RPS5Ap-mito

Plasmid

#163145

Purpose

Plant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter

Depositor

Shin-ichi Arimura

Article

Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

Insert

RPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG

Expression

Plant

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Elafin

Plasmid

#18102

Depositor

Ronny Drapkin

Article

Labidi-Galy et al Oncogene. 2015 Jan 15;34(3):373-83. doi:

Insert

Elafin (PI3 Human)

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pJOP-HTT-HR18Q

Plasmid

#87228

Purpose

HTT HR arms with 18 CAG repeats and piggyBac selection cassette

Depositor

Mahmoud Pouladi

Article

Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

Insert

1.7kb HTT 5' homology arm, 2.5kb HTT 3' homology arm

Expression

Mammalian

Promoter

EF1a driving selection cassette

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pEcRNAP6

Plasmid

#128940

Purpose

pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z

Depositor

Seth Darst

Article

Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

Inserts

RpoA (rpoA E. coli)
RpoB
RpoC-ppx(His)10
RpoZ

Tags

His

Expression

Bacterial

Mutation

PreScission protease site (GE Healthcare; LEVLFQG…

Promoter

T7

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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TALED_Left-mouse-ATP6-1397N.

Plasmid

#212951

Purpose

Expresses TALED_Left-mouse-ATP6-1397N in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Left-mouse-ATP6-1397N (ATP6 Synthetic)

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Right-Cox3.1-1397C-AD.

Plasmid

#212953

Purpose

ExpressesTALED_Right-Cox3.1-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-Cox3.1-1397C-AD

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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