We narrowed to 12,266 results for: cel.2

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pCDNA3-SREBP-1 (A341; A1171)

Plasmid

#204197

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1a (SREBF1 Human)

Tags

E-tag and T7x2

Expression

Mammalian

Mutation

Gly to Ala 341, 1171

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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B2M-SDMutation-gRNA

Plasmid

#215547

Purpose

gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

B2M (B2M Human)

Use

CRISPR

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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INS-3'gRNA

Plasmid

#210468

Purpose

gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

INS (INS Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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tetO-ZFPM2

Plasmid

#170707

Purpose

doxycycline-inducible overexpression of ZFPM2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

ZFPM2 (ZFPM2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

3 nonsynonymous changes: 353C>T (Ser>Phe)…

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pROS_phleo-Tps1/Gsy2

Plasmid

#196612

Purpose

Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

TPS1, GSY2 (TPS1, GSY2 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-ChRWR/Venus(ATG mut)

Plasmid

#197891

Purpose

Can be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

ChRWR/Venus

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pUGP1.1

Plasmid

#196615

Purpose

Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-Y373C

Plasmid

#191754

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-Y373C mutation (associated with TD1) (target mutation: c.1118A>G ; silent mutation added on purpose: c.1110C>A)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1118A>G, p.Y373C + c.1110C>A (silent muta…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-1

Plasmid

#190095

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-1 (ABHD2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

deleted the base pairs 6704-6709 refer to NM_0070…

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR207-MUM2sp-Citrine-ADPG2

Plasmid

#170725

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)

Use

Gateway donor vector / entry clone

Tags

Citrine tag (714bp)

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Zyxin-6

Plasmid

#55101

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Zyxin (ZYX Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-Puro-CLDN18-ARHGAP26

Plasmid

#69465

Purpose

Expresses Cldn18-Arhgap26 fusion gene.

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

CLDN18-ARHGAP26 (ARHGAP26, CLDN18 Human)

Use

Retroviral

Tags

HA TAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-Puro-CLEC16A-EMP2

Plasmid

#69468

Purpose

Expresses CLEC16A-EMP2 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

CLEC16A-EMP2 (CLEC16A, EMP2 Human)

Use

Retroviral

Tags

HA TAG

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

Plasmid

#197892

Purpose

Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

hM4D/2A/GFP

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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LAMP-mCherry-CRY2

Plasmid

#102249

Purpose

Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

LAMP-mCherry-CRY2 (LAMP1, CRY2 Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]

Plasmid

#184380

Purpose

The mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoter

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Myod1 (Myod1 Mouse)

Use

Lentiviral

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

Plasmid

#229795

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor

Plasmid

#229789

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mNgn2; ZF10-NS3-p65 (Neurog2 Mouse)

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF10 and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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nAChR beta2 YFP

Plasmid

#15107

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

beta2-YFP-M (Chrnb2 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pTetON-BMP2/7

Plasmid

#137913

Purpose

Inducible mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, divergent)

Depositor

Georg Feichtinger

Article

Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

Inserts

BMP2 (BMP2 Human)
BMP7 (BMP7 Human)

Expression

Mammalian

Promoter

TRE

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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