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We narrowed to 13,841 results for: Cre

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Showing: 6241 - 6260 of 13841 results

  1. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid
    #132962
    Purpose
    Firefly-luciferase single-strand annealing assay cloning backbone
    Depositor
    Gang Bao
    Publication
    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Firefly Luciferase
    Use
    CRISPR and Luciferase
    Expression
    Mammalian
    Mutation
    Stop sequence between luciferase gene
    Promoter
    CMV
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    132962-plasmid-map-sequence-id-259217

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  7. pAT9752-BEAR-mScarlet

    Plasmid
    #162991
    Purpose
    BEAR target plasmid with split mScarlet and disrupted 5' splice site
    Depositor
    Ervin Welker
    Publication
    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet split with an intron between amino acids 110-111
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    disrupted 5' splice site
    Promoter
    CMV
    Available since
    Sept. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162991-plasmid-map-sequence-id-324078

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  9. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Publication
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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  10. GAMA-bio

    Plasmid
    #47747
    Purpose
    Expresses enzymatically monobiotinylated full-length GAMA ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised GAMA
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47747-plasmid-map-sequence-id-68877

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  12. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234454
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Publication
    Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi
    Insert
    iGluSnFR4f-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234454-plasmid-map-sequence-id-465691

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  14. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

    Plasmid
    #169182
    Purpose
    Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Publication
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6-3xFlag
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169182-plasmid-map-sequence-id-333999

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  15. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139091
    Purpose
    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139091-plasmid-map-sequence-id-273888

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  17. Dom neg CstF64

    Plasmid
    #127250
    Purpose
    Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation
    Depositor
    Christine Milcarek
    Publication
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CstF64 delta 282 (CSTF2 Human)
    Use
    Other
    Expression
    Mammalian
    Mutation
    insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…
    Promoter
    pEF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127250-plasmid-map-sequence-id-250029

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  18. pcDNA5FRT/TO-Ataxin3-Strep-HA

    Plasmid
    #113496
    Purpose
    Expression of human Ataxin-3 with C-terminal strep-HA tag
    Depositor
    Hemmo Meyer
    Publication
    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ataxin-3 (ATXN3 Human)
    Tags
    2xstrep-HA
    Expression
    Mammalian
    Mutation
    wildtype without stop codon
    Promoter
    CMV
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113496-plasmid-map-sequence-id-218585

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Showing: 6241 - 6260 of 13841 results