We narrowed to 17,134 results for: Por

1. pDONR221_SLC22A2

   Plasmid

   #131964

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

   Insert

   SLC22A2 (SLC22A2 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. LOV-Turbo-V5_pRS415

   Plasmid

   #199667

   Purpose

   expresses LOV-Turbo in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Tags

   V5

   Expression

   Yeast

   Promoter

   Gal1,10

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLR5-CAG-NLS-SNAP

   Plasmid

   #174879

   Purpose

   PiggyBac transposon vector for expression of NLS-SNAP

   Depositor

   Hiroshi Ochiai

   Article

   Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

   Insert

   NLS-SNAP
   

   Use

   Piggybac vector

   Expression

   Mammalian

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLYS5-MCU-Flag

   Plasmid

   #50054

   Purpose

   Expression of human MCU in mammalian cells

   Depositor

   Vamsi Mootha

   Article

   Sancak et al Science. 2013 Nov 14.

   Insert

   MCU-Flag (MCU Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-UBC-H2-Kb SCT-T2A-GFP

   Plasmid

   #205454

   Purpose

   lentiviral plasmid for expression of mouse H2-Kb SCT

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   H2-Kb SCT (H2-K1 Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAL175

   Plasmid

   #22276

   Purpose

   Expresses YFP-PIF for red-light-induced binding to Phy

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   mYFP

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRX-IP/SECFP-hATG9A

   Plasmid

   #58995

   Purpose

   Expresses SECFP-hATG9A in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   Homo sapiens autophagy related 9A (PPARG Human)
   

   Use

   Retroviral

   Tags

   Venus

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57 APEX

   Plasmid

   #40306

   Purpose

   A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

   Insert

   pea APEX (Synthetic)
   

   Use

   Unspecified

   Mutation

   K14D, W41F, E112K (relative to wild-type)

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-mNectarine

   Plasmid

   #21717

   Purpose

   Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

   Depositor

   Robert Campbell

   Article

   Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

   Insert

   mNectarine fluorescent proteins
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Available Since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-mEGFP-PKCa-C1

    Plasmid

    #112264

    Purpose

    ITRACKalpha Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase C alpha (PRKCA Bovine)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    A11V (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcpBpaRS

    Plasmid

    #196484

    Purpose

    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    

    Expression

    Mammalian

    Promoter

    CMV (synthetase), Pol III tRNA promoter (tRNA)

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry

    Plasmid

    #177182

    Purpose

    plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    pegRNA targeting the PEAR-GFP plasmid
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Barcode backbone

    Plasmid

    #158665

    Purpose

    This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    hcRED

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nanog-2A-mCherry

    Plasmid

    #48680

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Nanog (Nanog Mouse)
    

    Use

    CRISPR

    Tags

    Cherry

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Trak2

    Plasmid

    #127622

    Purpose

    N-terminal GFP-tagged mouse Trak2

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak2 (Trak2 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTight-9-124

    Plasmid

    #31874

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)
    

    Use

    Lentiviral

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRXIB-mRuby3-STX17TM

    Plasmid

    #221744

    Purpose

    Stable expression of mRuby3-STX17TM in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    STX17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-228

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-Puro-RhoA FLARE.sc Biosensor

    Plasmid

    #12602

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

    Plasmid

    #216253

    Purpose

    FRET-based calcium sensor located in the ER lumen, Tet-inducible

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    ER-targeted D4 second generation cameleon (FRET calcium sensor)
    

    Use

    Lentiviral

    Tags

    REAch and mTurquoise

    Promoter

    TRE3G

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid

    #132962

    Purpose

    Firefly-luciferase single-strand annealing assay cloning backbone

    Depositor

    Gang Bao

    Article

    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

    Insert

    Firefly Luciferase
    

    Use

    CRISPR and Luciferase

    Expression

    Mammalian

    Mutation

    Stop sequence between luciferase gene

    Promoter

    CMV

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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