We narrowed to 6,523 results for: sas

1. pPB-EF1A-Puro-hTBK1-E696K-EGFP

   Plasmid

   #221554

   Purpose

   Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Piggybac

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed glutamate 696 to lysine

   Promoter

   EF1a

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL0284 (pQCascade_entry)

   Plasmid

   #130635

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCh-LgBiT-2A-EGFP-Vin-PILATeS

   Plasmid

   #216761

   Purpose

   PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pADSL-EGFP

   Plasmid

   #99109

   Purpose

   Expresses human ADSL-EGFP fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   An et al Science. 2008 Apr 4;320(5872):103-6. doi

   Insert

   ADSL (ADSL Human)
   

   Tags

   EGFP (enhanced green fluorescent protein)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAF123

   Plasmid

   #135921

   Purpose

   All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

   Depositor

   Andrew S. Flies

   Article

   Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Available Since

   Jan. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-Hyg-Dest-mCherry-PAR-6B

   Plasmid

   #194908

   Purpose

   To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

   Depositor

   Clare Buckley

   Article

   Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

   Insert

   PAR-6B (Pard6b Mouse)
   

   Use

   Mouse Targeting

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   39 C to G, silent mutation

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGST2-GST-TEV-OPTN

   Plasmid

   #198428

   Purpose

   Used for the expression and purification of OPTN (WT) (SMC Internal No. 1587)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   optineurin (OPTN Human)
   

   Tags

   GST and TEV

   Expression

   Bacterial

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTXB1-TP3

   Plasmid

   #160086

   Purpose

   Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase/APEX2 peroxidase fusion protein
   

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTXB1-TP5

   Plasmid

   #160088

   Purpose

   Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase/APEX2 peroxidase fusion protein
   

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid

    #130640

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-3xCTCF-TetO

    Plasmid

    #213023

    Purpose

    piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    3xCTCF-TetO
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid

    #169658

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

    Plasmid

    #201831

    Purpose

    piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Inserts

    CAG
    BFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-HuC:Dendra2

    Plasmid

    #80904

    Purpose

    Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

    Insert

    Dendra2 (elavl3 Synthetic)
    

    Use

    Zebrafish expression

    Promoter

    HuC

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-FKBP-GFP-NAP1

    Plasmid

    #208862

    Purpose

    Stably express GFP-tagged NAP1 for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

    Plasmid

    #169921

    Purpose

    DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.

    Depositor

    Zhili Rong

    Article

    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

    Insert

    IRES-Puro-pEF1a-EGFP
    

    Use

    CRISPR

    Promoter

    EF1a

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-p62-LIR-mutant (LIRAAAA)

    Plasmid

    #187981

    Purpose

    Plasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    DDDW335-338AAAA (LIR mutant), silent mutation siR…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-empty-DSE-TetO-8kb

    Plasmid

    #213022

    Purpose

    piggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    TetO
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_puro_CAG_HexaPro

    Plasmid

    #164077

    Purpose

    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus

    Depositor

    Yannick Doyon

    Article

    Rivest et al protocols.io 2021

    Insert

    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    CRISPR

    Tags

    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CAG

    Available Since

    Jan. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

    Plasmid

    #130639

    Purpose

    Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on Cas8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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