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gag-COM-pol

Plasmid

#211373

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-COM-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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cpLOV2-V7

Plasmid

#168993

Purpose

circularly permuted LOV2 variant-V7

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

cpLOV2-V7

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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cpLOV2-V5

Plasmid

#168991

Purpose

circularly permuted LOV2 variant-V5

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

cpLOV2-V5

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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gag-PE

Plasmid

#211371

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-PE

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Tags

anti-mouse nanobody

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-DFCP1

Plasmid

#38269

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Zinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pDAC435

Plasmid

#195238

Purpose

Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTW199b2

Plasmid

#136928

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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gag-MCP-pol

Plasmid

#211370

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-MCP-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRAM24

Plasmid

#234313

Purpose

pBBR1 origin cat-RNA U1376

Depositor

James Chappell

Article

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

U1367 Targeting RAM, Barcode 1

Use

Synthetic Biology

Expression

Bacterial

Promoter

P. CymRC

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pNP1950

Plasmid

#222729

Purpose

J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

Plasmid

#209294

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

SOD2-3xHA-TALE(RNR2-Left)-MutH

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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DL017:CMV::HaloTag-iPAK4

Plasmid

#177882

Purpose

HT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

iPAK4

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Expression

Bacterial

Mutation

see manuscript

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP

Plasmid

#235573

Purpose

Dox-inducible expression of control superfolder (sf)GFP fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

superfolder (sf)fGFP

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pUCIDT-AMP-Fp16SrRNA

Plasmid

#218519

Purpose

pUCIDT-AMP containing 16S rRNA gene of F. prausnitzii for absolute concentration quantification

Depositor

Ami Bhatt

Article

Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

Insert

16S rRNA

Use

Synthetic Biology

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_StrepII_TEV_PP2Ac

Plasmid

#217477

Purpose

Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag

Depositor

Wolfgang Peti

Article

Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

Insert

PP2Ac

Tags

StrepII, TEV

Expression

Mammalian

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PEmax-P2A-EGFP

Plasmid

#240534

Purpose

For lentiviral expression of PEmax with EGFP

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

PEmax-P2A-EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHCMV-SS-antiFLAGscFv-TM

Plasmid

#198303

Purpose

Displays anti-FLAG scFv from the surface of mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pHCMV-SS-antiFLAGscFv-TM

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Plasmid

#209296

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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