161,570 results

1. pAAV hSyn-fDIO-taCasp3-TEVp

   Plasmid

   #207997

   Purpose

   Inducing apoptotic ablation of neurons through a process reliant on Flp recombinase

   Depositor

   Kazunari Miyamichi

   Article

   Goto et al Neuron. 2025 Feb 17:S0896-6273(25)00041-

   Insert

   taCasp3-TEVp
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNIR-FbGFP

   Plasmid

   #184675

   Purpose

   NIR-Fb to GFP

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-FbGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEA186

   Plasmid

   #187874

   Purpose

   Empty pVS1-based INTEGRATE vector with mCherry cargo

   Depositor

   Kan Wang

   Article

   Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

   Plasmid

   #118154

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a core and U6

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-NLS-HA-TurboID

   Plasmid

   #215075

   Purpose

   Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   TurboID (birA )
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-LC3 (human)

   Plasmid

   #24920

   Purpose

   Mammalian expression of LC3 fused to EGFP

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   LC3 (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmU6-gRNA

   Plasmid

   #53187

   Purpose

   Expresses the S. pyogenes sgRNA from the mouse U6 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBPGUw

   Plasmid

   #17575

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Tools for neuroanatomy and neurogenetics

   Insert

   GAL4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI neo-hEST2-HA

    Plasmid

    #1782

    Depositor

    Bob Weinberg

    Article

    Meyerson et al Cell 1997 Aug 22;90(4):785-95.

    Insert

    hTERT (TERT Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Clone is catalytically active but is dead in vivo.

    Promoter

    Available since

    July 10, 2005

    Availability

    Academic Institutions and Nonprofits only