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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,832 results for: grna

Showing: 9451 - 9480 of 15832 results

  1. HCP4

    Plasmid
    #166106
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166106-plasmid-map-sequence-id-330381

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  2. pNSU299_RPL39

    Plasmid
    #166078
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166078-plasmid-map-sequence-id-330349

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  3. HCP6

    Plasmid
    #166108
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166108-plasmid-map-sequence-id-330318

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  4. pNSU299_YDR344C

    Plasmid
    #166072
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166072-plasmid-map-sequence-id-329603

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  5. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

    Plasmid
    #159907
    Purpose
    Mutagenesis of Netrin1
    Depositor
    Larry Zweifel
    Insert
    Netrin1 (Ntn1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159907-plasmid-map-sequence-id-317313

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  6. pTP63.1.0-gDNA

    Plasmid
    #132432
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TP63 (TP63 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132432-plasmid-map-sequence-id-266195

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  7. pCREB5.1.0-gDNA

    Plasmid
    #132475
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    CREB5 (CREB5 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132475-plasmid-map-sequence-id-267192

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  8. pESRRG.1.0-gDNA

    Plasmid
    #132473
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ESRRG (ESRRG Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132473-plasmid-map-sequence-id-267180

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  9. pGABPB2.1.0-gDNA

    Plasmid
    #132457
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    GABPB2 (GABPB2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    132457-plasmid-map-sequence-id-266980

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  10. pDLX4.1.0-gDNA

    Plasmid
    #132437
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    DLX4 (DLX4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132437-plasmid-map-sequence-id-266219

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  16. CIRTS-FTO-control

    Plasmid
    #216859
    Purpose
    CIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100
    Insert
    CIRTS-FTO-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    mCherry2
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216859-plasmid-map-sequence-id-430696

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  17. pYEE

    Plasmid
    #159750
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159750-plasmid-map-sequence-id-313819

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  24. AAV-KrasWT/sgKras/Cre

    Plasmid
    #99848
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template that serves as a control for HDR into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Promoter
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99848-plasmid-map-sequence-id-193786

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  25. pTEE

    Plasmid
    #159748
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159748-plasmid-map-sequence-id-313816

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Showing: 9451 - 9480 of 15832 results