We narrowed to 6,879 results for: crispr cas9 plasmids

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pLP110_AAV Scramble Control

Plasmid

#239419

Purpose

Negative control AAV vector for SaCas9-based CRISPR KO

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

Scramble control

Use

AAV

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCRIS

Plasmid

#120424

Purpose

This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

Depositor

Tamás Fehér

Article

Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

Insert

CRISPR spacers targeting IS1, IS5, IS3, IS150

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

constitutive

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-ARL13B

Plasmid

#227276

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of ARL13B (ARL13B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-TOMM20

Plasmid

#207789

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-GOLGA2

Plasmid

#207791

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-LAMP1

Plasmid

#207787

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-CANX

Plasmid

#227279

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CANX (CANX Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-VIM

Plasmid

#227301

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of VIM (VIM Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-CEP135

Plasmid

#227286

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CEP135 (CEP135 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CLTC

Plasmid

#227312

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CLTC (CLTC Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-RAB7A

Plasmid

#227297

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of RAB7A (RAB7A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-TP53

Plasmid

#227318

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of TP53 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-MAP4

Plasmid

#227295

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MAP4 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of MAP4 (MAP4 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-EZR

Plasmid

#227293

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of EZR (EZR Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CEP192

Plasmid

#227288

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CEP192 (CEP192 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-TRE3G-TdTomato

Plasmid

#192658

Purpose

A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, TRE3G

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGH224_sgRNA_2xMS2_Puro

Plasmid

#85413

Purpose

hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-miniCMV-TdTomato

Plasmid

#192656

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
7i sgRNA for 4-μHOM reporter

Plasmid

#113626

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7i sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only