We narrowed to 6,520 results for: sas;

1. pCAG_WIPI2d-TEV-GST

   Plasmid

   #223799

   Purpose

   Expression of recombinant protein for purification

   Depositor

   James Hurley, Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   TEV-GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-EF1A-HALO-hLRRK2

   Plasmid

   #229738

   Purpose

   Expresses Halo tagged human LRRK2 in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWT-IFl_WT-IFr

   Plasmid

   #192207

   Purpose

   Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Wildtype I-F Leader and wildtype I-F Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScr-IFl_WT-IFr

   Plasmid

   #192208

   Purpose

   Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Scrambled I-F Leader and wildtype I-F Locus DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMV-hAT-13_AMi

   Plasmid

   #217101

   Purpose

   Mammalian expression vector for expression of transposase (CMV promoter)

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   TIRs of hAT-13_AMi transposon
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMV-hAT-12_AMi

   Plasmid

   #217102

   Purpose

   Mammalian expression vector for expression of transposase (CMV promoter)

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   TIRs of hAT-12_AMi transposon
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMV-Mariner-7_Croc

   Plasmid

   #217103

   Purpose

   Mammalian expression vector for expression of transposase (CMV promoter)

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   TIRs of Mariner-7_Croc transposon
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

   Plasmid

   #208872

   Purpose

   For expression in E. coli of NAP1 (delta-NDP52)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   S37K/A44E (disrupts NDP52 binding)

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

   Plasmid

   #208873

   Purpose

   For expression in E. coli of NAP1 (delta-TBK1)

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   L226Q/L233Q (disrupts TBK1 binding)

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)

    Plasmid

    #208863

    Purpose

    Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    S37K/A44E (disrupts NDP52 binding)

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)

    Plasmid

    #208864

    Purpose

    Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    I11S/L12S (disrupts FIP200 binding)

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)

    Plasmid

    #208865

    Purpose

    Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    L226Q/L233Q (disrupts TBK1 binding)

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

    Plasmid

    #198037

    Purpose

    Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST-TEV-EGFP

    Expression

    Insect

    Mutation

    E696K

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac-GST-TEV-mCherry-TBK1

    Plasmid

    #198033

    Purpose

    Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    GST-TEV and mCherry

    Expression

    Insect

    Mutation

    E696K

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Duet1-6xHis-TEV-OPTN

    Plasmid

    #190192

    Purpose

    Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid

    #169657

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid

    #169659

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE2-Ef1a-SOX2-3xFLAG

    Plasmid

    #216197

    Purpose

    Overexpress human SOX2 in HEK 293T cells for use in whole cell extract EMSAs

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid

    #221002

    Purpose

    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25-IRES-BleoR
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LiOn-CAG∞RFP

    Plasmid

    #154016

    Purpose

    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

    Depositor

    Jean Livet

    Article

    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

    Insert

    mRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H210P

    Promoter

    CAG

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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