162,717 results

1. PAP-delta10-cHIS

   Plasmid

   #21716

   Depositor

   Andrew Bohm

   Article

   Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

   Insert

   polyA polymerase (PAP1 Budding Yeast)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   30 amino acids off the c terminus

   Promoter

   Available since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

2. PASTE payload acceptor with attP site pDY2087

   Plasmid

   #219859

   Purpose

   Bxb1 payload vector with 5' attP

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pICSL60004

   Plasmid

   #117519

   Purpose

   rbcS-E9 terminator Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:GCTT:E9t:CGCT:BsaI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_T7-ARG1

   Plasmid

   #106473

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p5E-elavl3

   Plasmid

   #75025

   Purpose

   Can be used to drive pan neuronal expression in zebrafish.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Tags

   Expression

   Mutation

   Promoter

   elavl3

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. LZF43: Cre-on CAG-CheRiff-CFP (DIO)

   Plasmid

   #104114

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   CheRiff-CFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pB-MultiplexedReporter-MOR170-1

   Plasmid

   #129461

   Purpose

   piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   MOR170-1 (Olfr895 Mouse)
   

   Use

   Synthetic Biology

   Tags

   LUCY tag, Rho tag

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (Tet-On)

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EB3N-VVDfast-mCl3-iLID

   Plasmid

   #190165

   Purpose

   Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   EB3N-VVDfast-mClover3-iLID
   

   Use

   Tags

   -VVDfast-mClover3-iLID

   Expression

   Mammalian

   Mutation

   1-189 aa from EB3

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLM494

    Plasmid

    #100539

    Purpose

    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

    Inserts

    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1, TDH3, TIP1, and ZEO1

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LMAN1-bio-His

    Plasmid

    #51922

    Purpose

    Expresses full-length ERGIC-53 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LMAN1 (LMAN1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV/MASC_(GLuc)_TOP1

    Plasmid

    #68439

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX6-Smad3 WT

    Plasmid

    #27010

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pRK5-mEGFP-SQSTM1-WT

    Plasmid

    #194582

    Purpose

    Mammalian Expression of mEGFP-SQSTM1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    SQSTM1 (SQSTM1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDB4581

    Plasmid

    #171124

    Purpose

    A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

    Insert

    3_sAID
    

    Use

    Other

    Tags

    Expression

    Mutation

    non applicable

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

    Plasmid

    #116931

    Purpose

    Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    lumazine synthase
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Halo-Cyto

    Plasmid

    #124314

    Purpose

    Expresses cytosolic specific HaloTag protein

    Depositor

    Jin Wang

    Article

    Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

    Insert

    Halo tag
    

    Use

    Tags

    Cytoplasm specific seq

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBad33_ISTfu1_reRNA

    Plasmid

    #205355

    Purpose

    Bacterial expression of ISTfu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISTfu1 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TetON-shYAP

    Plasmid

    #115667

    Purpose

    Inducible YAP knockdown

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP shRNA (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge