Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

178,218 results

Showing: 6361 - 6380 of 178218 results

  3. cytoplasmic NAD+ biosensor

    Plasmid
    #186787
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm
    Depositor
    Michael Cohen
    Publication
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    cytoplasmic NAD+ biosensor
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    186787-plasmid-map-sequence-id-368056

    Enlarge

  4. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid
    #183532
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    183532-plasmid-map-sequence-id-362037

    Enlarge

  8. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171991-plasmid-map-sequence-id-338127

    Enlarge

  9. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

    Plasmid
    #162380
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV1, AAV9, and AAV Retrograde
    Insert
    jGCaMP8s
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    S26M F286Y Q315H
    Promoter
    CAG
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162380-plasmid-map-sequence-id-318379

    Enlarge

  10. pLenti-PalmGRET

    Plasmid
    #158221
    Purpose
    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.
    Depositor
    Charles P. Lai
    Publication
    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014
    Insert
    PalmGFP-Nluc
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158221-plasmid-map-sequence-id-309022

    Enlarge

  15. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Publication
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Expression
    Mammalian
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128605-plasmid-map-sequence-id-318004

    Enlarge

  17. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway Cloning
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122843-plasmid-map-sequence-id-244374

    Enlarge

Showing: 6361 - 6380 of 178218 results