176,482 results
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Plasmid#70649PurposeExpresses Fibrillarin nucleolar marker with TurboRFP reporter.DepositorPublicationAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pSS9
Plasmid#71655PurposeContains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coliDepositorPublicationInsertgfpuv
UseSynthetic BiologyPromoterT7A1Available sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKCcas9dO
Plasmid#62552PurposeHigh efficiency Streptomyces genome editing by CRISPR/Cas9 systemDepositorInsertsScocas9
sgRNA ()
act-orf4 homology arm
UseCRISPR; Bacterial genome editingExpressionBacterialPromoterj23119 and ptipAAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLNCX2-CFP-VGPDFGR-YFP
Plasmid#59587PurposeEncodes a granzyme B FRET reporterDepositorInsertECFP-VGPDFGR-Venus
UseRetroviralTagsECFP and VenusExpressionMammalianPromoterCMVAvailable sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-B
Plasmid#66190Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selectionDepositorInsertCas9
ExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MOS
Plasmid#64120PurposeA modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genesDepositorAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MP4
Plasmid#69652PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA
ExpressionBacterialPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
Plasmid#64219PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2ADepositorInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFP-P2A-E1BExpressionMammalianPromoterCBh and U6Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Emerin pEGFP-N2 (588)
Plasmid#61985Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNeuroD-ires-GFP
Plasmid#61403PurposeMammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequenceDepositorTypeEmpty backboneExpressionMammalianPromoterNeuroDAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60228PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianPromoterCBh and U6Available sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Occludin-N-10
Plasmid#55112PurposeLocalization: Tight Junctions, Excitation: 587, Emission: 610DepositorAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pLenti-EmGFP-LATS2/1 KD
Plasmid#52085PurposeLentiviral RNAi vector for knockdown of LATS2 and LATS1. Co-expresses EmGFP as reporterDepositorPublicationAvailable sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
TagsSpyCatcher and SpyTagExpressionBacterialPromoterT7 promoterAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p(HA)HIF1alpha (P402A,P564A)
Plasmid#52636Purposemammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutationDepositorInsertHIF1alpha P402A, P564A (HIF1A Human)
TagsHAExpressionMammalianMutationP402A, P564APromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PH-PLCD1(R40L)-GFP
Plasmid#51408PurposeNon-PI(4,5)P2 binding control for PH-PLCD1-GFPDepositorInsertPH-PLCD1 (aa 1–170) (PLCD1 Human)
TagsEGFPExpressionMammalianMutationArginine 40 mutated to leucineAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE-tdTomato
Plasmid#50798PurposeExpression of tdTomato under the control of the TRE-promoterDepositorInsertTREtight driven tdTomato fluorescent protein
UseMutli-speciesPromoterTREtightAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-hCas9
Plasmid#51142PurposeExpresses hCas9 under the CAG promoter for CRISPRDepositorInserthCas9
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterCAGAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by