We narrowed to 7,286 results for: 11

1. PaCasp7a

   Plasmid

   #166051

   Purpose

   a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase

   Depositor

   Clay Clark

   Article

   Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

   Insert

   Porites astreiodes caspase-7a
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AlkB-D135G

   Plasmid

   #160434

   Purpose

   A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly

   Depositor

   Tao Pan

   Article

   Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

   Insert

   E. coli AlkB
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 135 to Glycine; deleted the…

   Promoter

   T7

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14

   Plasmid

   #162820

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_ORF9c/14
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   CoV2_ORF9c/14(Met1,Amber)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

   Plasmid

   #162822

   Purpose

   STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-(N-TAG)CoV2_ORF3b(CoV1h)
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   CoV2_ORF3b(CoV1h)(Met1,Amber)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b-ecDHFR-dCys

   Plasmid

   #110541

   Purpose

   Bacterial protein of E.coli DHFR C85A/C152S mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   C85 mutated to A85, C152 mutated to S152

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS315-PGK-SEIPIN2

   Plasmid

   #96987

   Purpose

   Express GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

   Insert

   SEIPIN2 (AT1G29760 Mustard Weed)
   

   Expression

   Yeast

   Promoter

   Phosphoglycerate Kinase (PGK) promoter

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDC84-SEIPIN3N-1C

   Plasmid

   #96983

   Purpose

   Express mutated Arabidopsis SEIPIN1 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

   Insert

   SEIPIN1 (AT5G16460 Mustard Weed)
   

   Tags

   6xHis and GFP

   Expression

   Plant

   Mutation

   A segment of 507 nucleotides from the N terminus…

   Promoter

   35S

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMDC84-SEIPIN1N-3C

   Plasmid

   #96982

   Purpose

   Express mutated Arabidopsis SEIPIN3 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

   Insert

   SEIPIN3 (AT2G34380 Mustard Weed)
   

   Tags

   6xHis and GFP

   Expression

   Plant

   Mutation

   A segment of 108 nucleotides from the N terminus…

   Promoter

   35S

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a - MPX

   Plasmid

   #89619

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Mitocondrial Tryparedoxin peroxidase
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a - EtfA

    Plasmid

    #89616

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Electron transport flavoprotein alpha subunit
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a - EtfB

    Plasmid

    #89617

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    electron-transfer-flavoprotein, alpha polypeptide
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a - TDR

    Plasmid

    #89627

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    thiol dependent reductase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a - NFDD

    Plasmid

    #89624

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD/FAD dependent dehydrogenase
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK516

    Plasmid

    #71578

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to glutamine

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK524

    Plasmid

    #71580

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes asparagine-347 to alanine

    Promoter

    T7

    Available Since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK513

    Plasmid

    #71576

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-294 to alanine

    Promoter

    T7

    Available Since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET24a_CDP-CHase

    Plasmid

    #73161

    Purpose

    Expresses in E. coli, Bacillus cereus NP_831800 CDP-CHase

    Depositor

    Maurice Bessman, Sandra Gabelli

    Article

    Duong-Ly et al J Bacteriol. 2011 Jul;193(13):3175-85. d

    Insert

    pET24a
    

    Expression

    Bacterial

    Available Since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pJK564

    Plasmid

    #71581

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes serine-71 to alanine

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK517

    Plasmid

    #71579

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to aspartate

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK514

    Plasmid

    #71577

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamine-435 to alanine

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge