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Kv7.1/pcDNA3.1

Plasmid

#111452

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)

Use

Tags

8xHis and EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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T7-p53DD-pcDNA3

Plasmid

#25989

Depositor

William Kaelin

Article

Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

Insert

tumor protein p53 (TP53 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

truncated cDNA encodes truncated protein (aa 300-…

Promoter

Available since

Oct. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TAK1/F

Plasmid

#44161

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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HA-USPL1-pcDNA3.1

Plasmid

#85760

Purpose

mammalian expression of full length HA tagged USPL1

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, full length (USPL1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IKZF1-V2-V5

Plasmid

#107390

Purpose

Mammalian expression of IKZF1-V2-V5

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V2 (IKZF1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2

Plasmid

#224769

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mApple_pcDNA3.1+

Plasmid

#182855

Purpose

Monomer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3. Flag-CSL R192H

Plasmid

#167005

Purpose

Mammalian expression of CSL R192H

Depositor

Gian-Paolo Dotto

Article

Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

Insert

CSL (RBPJ Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R192H

Promoter

CMV

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-BirA*-KRASG12V

Plasmid

#122208

Purpose

Expresses myc-BirA* fused to KRASG12V in mammalian cells

Depositor

Christopher Counter

Article

Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

Insert

KRASG12V (KRAS Human)

Use

Tags

myc-BirA*

Expression

Mammalian

Mutation

changed G12 to V

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A CARD8 CARD-FLAG WT

Plasmid

#164020

Purpose

Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-124

Plasmid

#26306

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-124 (MIR124-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

Plasmid

#181859

Purpose

Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

cp-PKN-mRuby2-GFP(1-0)

Use

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 βarr1 S412D Flag

Plasmid

#42196

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412D (Arrb1 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

S412D

Promoter

CMV

Available since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 βarr1 S412A Flag

Plasmid

#42195

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412A (Arrb1 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

S412A

Promoter

CMV

Available since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 CARD-FLAG WT

Plasmid

#163978

Purpose

Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-128

Plasmid

#26308

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-128 (MIR128-1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

Plasmid

#112842

Purpose

transient/stable overexpression of the central domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

C1 domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS W391A pcDNA3

Plasmid

#206077

Purpose

Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3. Flag-CSL Q307R

Plasmid

#167009

Purpose

Mammalian expression of CSL Q307R

Depositor

Gian-Paolo Dotto

Article

Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

Insert

CSL (RBPJ Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Q307R

Promoter

CMV

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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