2,294 results

1. miniCGBE1-NG (pBM1182)

   Plasmid

   #140257

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_NG-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE GFP-CACNA1A KI

   Plasmid

   #131480

   Purpose

   Endogenous tagging of CaV2.1, P/Q: N-terminal (amino acid position: G10)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cacna1a, Cacna1a Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-SypHluorin 2x (S2x)

   Plasmid

   #37004

   Depositor

   Yongling Zhu, Stephen Heinemann

   Article

   Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

   Insert

   SypHluorin 2x (Syp Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Two copies of pHluorin inserted at site 184-185

   Promoter

   CMV

   Available Since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HA-DNM2-R465W

   Plasmid

   #155262

   Purpose

   Mammalian expression of rat DNM2 fused to HA

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Arginine 465 to Tryptophan

   Available Since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MUNC18a

   Plasmid

   #218888

   Purpose

   Expresses rat MUNC18a in bacteria

   Depositor

   Axel Brunger

   Article

   Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

   Insert

   Munc18a (Stxbp1 Rat)
   

   Tags

   6 his

   Expression

   Bacterial

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBa.Halo-myosin Va tail 1006-1828

   Plasmid

   #162715

   Purpose

   Mammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residues

   Depositor

   Marvin Bentley

   Article

   Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

   Insert

   Myosin Va (Myo5a Rat)
   

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 1005 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA TrkB

   Plasmid

   #24088

   Depositor

   Moses Chao

   Article

   Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

   Insert

   TrkB (Ntrk2 Rat)
   

   Expression

   Mammalian

   Mutation

   R133I

   Available Since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGas-miRFP670nano

   Plasmid

   #127441

   Purpose

   G protein alpha-s internally tagged with miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   G protein alpha-s-miRFP670nano
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. μ2-HA-D176A

   Plasmid

   #32754

   Depositor

   Alexander Sorkin

   Article

   Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

   Insert

   μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D176A

   Promoter

   CMV

   Available Since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-T-rnVAPA-1-125

    Plasmid

    #13396

    Depositor

    Axel Brunger

    Article

    Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

    Insert

    VAP-A (Vapa Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

11. mCherry-RatLC3B-C-7

    Plasmid

    #55128

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    LC3B
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pMRX-IP-EGFP-PA-BAX(mito)

    Plasmid

    #228942

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAd-PV-NLS-DsRed

    Plasmid

    #17287

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-DsRed-NLS-CD (Pvalb Rat)
    

    Use

    Adenoviral

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

14. Cav2.1 HA pcDNA3

    Plasmid

    #206069

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HS-aPKC-CAAX-mKate2

    Plasmid

    #105950

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    aPKC-CAAX
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSAC5.02

    Plasmid

    #16077

    Depositor

    Jochen Buck, Lonny Levin

    Article

    Buck et al Proc Natl Acad Sci U S A. 1999 Jan 5. 96

    Insert

    soluble adenylyl cyclase (Adcy10 Rat)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

17. AAV-EF1a-jGCaMP8f-WPRE

    Plasmid

    #176756

    Purpose

    AAV-mediated expression of GCaMP8f under the EF1a promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid

    #107737

    Purpose

    In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.

    Depositor

    Rylan Larsen

    Insert

    synaptophysin-GCaMP6s, mRuby3
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. C1-F-tractin-mCitrine

    Plasmid

    #155217

    Purpose

    Monitor global F-actin distribution

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

    Plasmid

    #38262

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Tags

    Flag and PIP5K Iα core kinase domain (residues 18…

    Expression

    Mammalian

    Mutation

    Arg 233, Arg237, and Lys251 converted to Glutamin…

    Promoter

    CMV

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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