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Showing: 1241 - 1280 of 2070 results

  1. pAA3

    Plasmid
    #154163
    Purpose
    Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    20 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154163-plasmid-map-sequence-id-309398

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  3. pEND-321_Cacnes_ori

    Plasmid
    #225603
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225603-plasmid-map-sequence-id-451386

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  4. pEND-443_Cacnes_HisB

    Plasmid
    #225608
    Purpose
    Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225608-plasmid-map-sequence-id-451366

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  5. pEND-446_Cacnes_ProA

    Plasmid
    #225609
    Purpose
    Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225609-plasmid-map-sequence-id-451370

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  6. pEND-320_Cacnes_ori

    Plasmid
    #225602
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225602-plasmid-map-sequence-id-451377

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  11. pKM5

    Plasmid
    #154166
    Purpose
    Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Rel2 (long isoform)
    Use
    Tags
    FLAG
    Expression
    Insect
    Mutation
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154166-plasmid-map-sequence-id-309405

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  12. pKM28

    Plasmid
    #154165
    Purpose
    Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Rel1A
    Use
    Tags
    FLAG
    Expression
    Insect
    Mutation
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154165-plasmid-map-sequence-id-309403

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  13. pAA2

    Plasmid
    #154162
    Purpose
    Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    16 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154162-plasmid-map-sequence-id-309382

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  14. pAA1

    Plasmid
    #154161
    Purpose
    Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    12 wt NF-κB sites were replaced with Rel1A-specif…
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154161-plasmid-map-sequence-id-309344

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  15. pKM62

    Plasmid
    #154160
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    4 wt NF-κB sites were replaced with Rel2-specific…
    Promoter
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154160-plasmid-map-sequence-id-309343

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  16. pKM59

    Plasmid
    #154158
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Enhancer for the Drosophila melanogaster Metchnik…
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154158-plasmid-map-sequence-id-309332

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  17. pKM52

    Plasmid
    #154155
    Purpose
    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB site
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Drosophila melanogaster minimal Hsp70 promoter wi…
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154155-plasmid-map-sequence-id-309312

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  18. pKM51

    Plasmid
    #154154
    Purpose
    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Drosophila melanogaster minimal Hsp70 promoter wi…
    Available since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154154-plasmid-map-sequence-id-309306

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  23. pEND-227_Cacnes_mCerulean

    Plasmid
    #225614
    Purpose
    Replicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    mCerulean
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225614-plasmid-map-sequence-id-451417

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  25. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  27. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

    Plasmid
    #196677
    Purpose
    AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).
    Depositor
    Haining Zhong
    Article
    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:
    Insert
    PKIα-P2A-mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196677-plasmid-map-sequence-id-385124

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  28. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

    Plasmid
    #196678
    Purpose
    AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.
    Depositor
    Haining Zhong
    Article
    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:
    Insert
    PKIαmut-P2A-mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196678-plasmid-map-sequence-id-385123

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  37. pEND-184_Cacnes_mCherry

    Plasmid
    #225610
    Purpose
    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225610-plasmid-map-sequence-id-451420

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Showing: 1241 - 1280 of 2070 results