We narrowed to 1,677 results for: tyr
-
Plasmid#58462PurposeExpresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
TagsmycExpressionMammalianMutationTyrosine 20 to alaninePromoterCMV IEAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pN1-Lck-GCaMP2
Plasmid#24794DepositorInsertLck-GCaMP2
TagsHis and XpressExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…Available SinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT6-H133Y-FLAG
Plasmid#102324PurposeHuman SIRT6 H133Y with FLAG tag for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
TagsFLAGExpressionMammalianMutationchanged histidine 133 to tyrosinePromoterCMVAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE-30-RexYFP
Plasmid#141070PurposeBacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratioDepositorInsertRexYFP
Tags6xHisExpressionBacterialMutationchanged Leucine 169 to Proline, changed Tyrosine …PromoterT5Available SinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δrev
Plasmid#101348PurposeAll mutations in rev are silent with respect to the tat reading frame. Start ATG->ACG and nts 68-71 were mutated AGC->TCA to change tyrosine to a stop.DepositorInsertNL4-3
UseLentiviralMutationdelta revAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
TagsHis and TEVExpressionBacterialAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-5102HRasCT(Picchu)
Plasmid#209917PurposeA FRET biosensor for tyrosine kinase activityDepositorInsert5102HRasCT
TagsYPet and nTurquoise-GLExpressionMammalianAvailable SinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 GABARAP WT
Plasmid#123080PurposeGateway entry clone encoding human GABARAP wild-typeDepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseGateway entry vector / entry cloneAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sik-WT
Plasmid#210722PurposeConstruct for stable expression of mouse wild type Ptk6 (Sik) in mammalian cells.DepositorInsertProtein tyrosine kinase 6 (Ptk6 Mouse)
ExpressionMammalianAvailable SinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-2xFYVEhgs
Plasmid#109505PurposeMuscle specific zebrafish expression of mKate2 fused to mouse 2x PH domain from HGF regulated tyrosine kinase substrate (Hgs). Binds PtdIns3PDepositorInsertactc1b-mKate2-2xFYVEhgs
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbsr-6085x
Plasmid#108653PurposeA tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInsertPicchuEV
UseLentiviralTagsXFPExpressionMammalianAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCT
Plasmid#60620PurposePlasmid encoding iCas9 and tracrRNA on pRS415 backboneDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98N-pBabe-puro
Plasmid#19236DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 98 to AsparaginAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
ExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS423-FjTAL(JBEI-15466)
Plasmid#87936PurposeExpression in S.cerevisiae of FjTALDepositorInsertFjTAL
ExpressionYeastAvailable SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-Y1699C
Plasmid#25084DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationTyrosine 1699 mutated to CysteineAvailable SinceJuly 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y112N-pBabe-puro
Plasmid#19238DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 112 to AsparaginAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y112H-pBabe-puro
Plasmid#19237DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 112 to HistidinAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2
Plasmid#111269Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
ExpressionBacterialPromoterT7Available SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR-Blunt II-TOPO-PTPRD
Plasmid#29705DepositorInsertProtein tyrosine phosphatase receptor-type delta (PTPRD Human)
UseCloning vectorMutationnoneAvailable SinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98H-pBabe-puro
Plasmid#19235DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 98 to HistidinAvailable SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
actc1b-BTK-mKate2
Plasmid#109501PurposeMuscle specific zebrafish expression of mKate2 fused to the PH domain from Human Bruton tyrosine kinase (BTK). Binds PtdIns(3,4,5)P3.DepositorInsertactc1b-BTK-mKate2
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 FYN
Plasmid#67106PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-G1707R
Plasmid#32526DepositorInsertProtein tyrosine phosphatase receptor-type delta G1707R (PTPRD Human)
UseLentiviralExpressionMammalianMutationG1707RAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-P1690F
Plasmid#32525DepositorInsertProtein tyrosine phosphatase receptor-type delta P1690F (PTPRD Human)
UseLentiviralExpressionMammalianMutationP1690FAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
QuasAr-P2A-GCaMP
Plasmid#154082PurposeIndependent expression of QuasAr and GCaMP under human synapsin 1 promoterDepositorInsertQuasAr-P2A-GCaMP6f
UseAAVExpressionMammalianMutationChanged Tyrosine 327 to AlaninePromoterhuman synapsin 1Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
ARL13B-(Y86C)-GFP
Plasmid#246840PurposeExpress Arl13B (resistance to sgRNA), tagged with GFP, with Y86C mutationDepositorInsertArl13B (ARL13B Human)
UseLentiviralTagsGFPExpressionMammalianMutationchanged Tyrosine 86 to CysteineAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ36-72
Plasmid#232060PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 36-72DepositorInsertNeurofilament-Heavy tail domain lacking residues 36-72
Tags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHΔ462-638
Plasmid#232061PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638DepositorInsertNeurofilament-Heavy tail domain lacking residues 462-638
Tags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFMtail
Plasmid#232056PurposeExpresses construct for surface tethering: R. norvegicus NFM tail domainDepositorInsertNeurofilament-Medium tail domain
Tags6xHis, thrombinExpressionBacterialMutationcDNA codon-optimized for E. coli; added N-termina…PromoterT7Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28-NFHtail
Plasmid#232057PurposeExpresses construct for surface tethering: R. norvegicus NFH tail domainDepositorInsertNeurofilament-Heavy tail domain
Tags6xHisExpressionBacterialMutationadded N-terminal tryptophan and tyrosine, and cys…Available SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAMC042z21
Plasmid#209747PurposeRepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL blaDepositorInsertsfGFP - tRNA operon
ExpressionBacterialMutationsfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y115S/Y132S/Y139S
Plasmid#216408PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutationsDepositorInsertEfg1-ΔDBD-Y115S/Y132S/Y139S
Tags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 115, 132 and 139 to Serine, and …Available SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRH2941
Plasmid#210143PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1 (ARO7 Budding Yeast)
ExpressionYeastAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y4S/Y8S/Y16S
Plasmid#216405PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutationsDepositorInsertEfg1-ΔDBD-Y4S/Y8S/Y16S
Tags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 4, 8 and 16 to Serine, and delet…Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y64S/Y66S/Y69S
Plasmid#216406PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutationsDepositorInsertEfg1-ΔDBD-Y64S/Y66S/Y69S
Tags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 64, 66 and 69 to Serine, and del…Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y101S/Y104S/Y106S
Plasmid#216407PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutationsDepositorInsertEfg1-ΔDBD-Y101S/Y104S/Y106S
Tags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 101, 104 and 106 to Serine, and …Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y401S/Y404S/Y409S
Plasmid#216409PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutationsDepositorInsertEfg1-ΔDBD-Y401S/Y404S/Y409S
Tags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 401, 404 and 409 to Serine, and …Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y450S/Y452S/Y456S
Plasmid#216410PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutationsDepositorInsertEfg1-ΔDBD-Y450S/Y452S/Y456S
Tags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 450, 452 and 456 to Serine, and …Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y478S/Y482S/Y484S
Plasmid#216411PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutationsDepositorInsertEfg1-ΔDBD-Y478S/Y482S/Y484S
Tags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 478, 482 and 484 to Serine, and …Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only