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Addgene

We narrowed to 4,232 results for: Gca

Showing: 1551 - 1600 of 4232 results
  1. pCAGGS-ROSAf

    Plasmid
    #54323
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGG
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pICSL4723_ERF922

    Plasmid
    #126883
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. 8061-E06

    Plasmid
    #234243
    Purpose
    Gateway ORF Entry clone of human TGFBR3 with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    TGFBR3 (TGFBR3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. LT3GEPIR-NEK9shRNA2

    Plasmid
    #220555
    Purpose
    To inducibly knockdown NEK9 expression
    Depositor
    Insert
    NEK9 shRNA2 (NEK9 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pCfb13220

    Plasmid
    #219895
    Purpose
    The base plasmid of TUNEYALI for TF30
    Depositor
    Insert
    Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pCfb13237

    Plasmid
    #219910
    Purpose
    The base plasmid of TUNEYALI for TF47
    Depositor
    Insert
    Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pCfb13242

    Plasmid
    #219914
    Purpose
    The base plasmid of TUNEYALI for TF52
    Depositor
    Insert
    Contains gRNA targeting TF52 (YALI1_E12668g) and homologous arm matching TF52
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pCfb13209

    Plasmid
    #219884
    Purpose
    The base plasmid of TUNEYALI for TF19
    Depositor
    Insert
    Contains gRNA targeting TF19 (YALI1_A20855g) and homologous arm matching TF19
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pCfb13233

    Plasmid
    #219906
    Purpose
    The base plasmid of TUNEYALI for TF43
    Depositor
    Insert
    Contains gRNA targeting TF43 (YALI1_C14774g) and homologous arm matching TF41
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pCfb13196

    Plasmid
    #219871
    Purpose
    The base plasmid of TUNEYALI forTF06
    Depositor
    Insert
    Contains gRNA targeting TF06 (YALI1_D34785g) and homologous arm matching TF06
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pCfb13217

    Plasmid
    #219892
    Purpose
    The base plasmid of TUNEYALI for TF27
    Depositor
    Insert
    Contains gRNA targeting TF27 (YALI1_E22758g) and homologous arm matching TF27
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pCfb13222

    Plasmid
    #219897
    Purpose
    The base plasmid of TUNEYALI for TF32
    Depositor
    Insert
    Contains gRNA targeting TF32 (YALI1_F33301g) and homologous arm matching TF32
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. pCfb13227

    Plasmid
    #219902
    Purpose
    The base plasmid of TUNEYALI for TF37
    Depositor
    Insert
    Contains gRNA targeting TF37 (YALI1_D01890g) and homologous arm matching TF37
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pCfb13216

    Plasmid
    #219891
    Purpose
    The base plasmid of TUNEYALI for TF26
    Depositor
    Insert
    Contains gRNA targeting TF26 (YALI1_D05594g) and homologous arm matching TF26
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. pCfb13229

    Plasmid
    #219904
    Purpose
    The base plasmid of TUNEYALI for TF39
    Depositor
    Insert
    Contains gRNA targeting TF39 (YALI1_B16808g) and homologous arm matching TF39
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. pCfb13205

    Plasmid
    #219880
    Purpose
    The base plasmid of TUNEYALI for TF15
    Depositor
    Insert
    Contains gRNA targeting TF15 (YALI1_B29562g) and homologous arm matching TF15
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. pCfb13221

    Plasmid
    #219896
    Purpose
    The base plasmid of TUNEYALI for TF31
    Depositor
    Insert
    Contains gRNA targeting TF31 (YALI1_D18727g) and homologous arm matching TF31
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. pCfb13248

    Plasmid
    #219919
    Purpose
    The base plasmid of TUNEYALI for TF58
    Depositor
    Insert
    Contains gRNA targeting TF58 (YALI1_F37742g) and homologous arm matching TF58
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid
    #201402
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Insert
    alpha-SMA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  20. SGEP-sh-Hs-PHGDH-1957

    Plasmid
    #188674
    Purpose
    shRNA
    Depositor
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. SGEP-sh-Hs-PHGDH-1967

    Plasmid
    #188672
    Purpose
    shRNA
    Depositor
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  22. mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177211
    Purpose
    Expresses a Neomycin-targeting gRNA (mU6), a non-targeting gRNA (hU6) and Cre-recombinase
    Depositor
    Insert
    sgNeo/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  23. pZNF253.1.0-gDNA

    Plasmid
    #132461
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Insert
    ZNF253 (ZNF253 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  24. pZNF780A.1.0-gDNA

    Plasmid
    #132446
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Insert
    ZNF780A (ZNF780A Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
  25. pZNF3.1.0-gDNA

    Plasmid
    #132440
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Insert
    ZNF3 (ZNF3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
  26. PX458_PBX2_1

    Plasmid
    #72357
    Purpose
    Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFP
    Insert
    PBX2 (PBX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  27. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  28. pHnS KI;Actb Donor;3xV5 KO;Template

    Plasmid
    #240291
    Purpose
    KI:Actb Donor:3xV5 KO:Template
    Depositor
    Insert
    KI gRNA for Actb
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  29. pHnS 2comp Donor;smFP-V5 KO;Gphn#1

    Plasmid
    #240307
    Purpose
    Donor:smFP-V5 KO:Gphn#1
    Depositor
    Insert
    KO gRNAs for Gphn
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  30. ipUSEPR-sg-Hs-CD4

    Plasmid
    #188691
    Purpose
    sgRNA
    Depositor
    Insert
    sgCD4 (CD4 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  31. ipUSEPR-sg-Hs-PHGDH-int2

    Plasmid
    #188682
    Purpose
    control sgRNA
    Depositor
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
  32. HCP7

    Plasmid
    #166109
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.
    Depositor
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  33. pRARB.1.0-gDNA

    Plasmid
    #132471
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Insert
    RARB (RARB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  34. pICSL4723_ Rac5

    Plasmid
    #126885
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  35. pENTRY4_Rac5

    Plasmid
    #126897
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
  36. pENTRY4_ OBfold

    Plasmid
    #126901
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  37. pICSL4723_ OBfold

    Plasmid
    #126889
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  38. pENTRY4_ ERF922

    Plasmid
    #126893
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  39. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Insert
    gRNA against mouse TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  40. bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

    Plasmid
    #177232
    Purpose
    Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgSik1_1st/sgSik2/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  41. bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2

    Plasmid
    #177231
    Purpose
    Expresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgNT1/sgNeo2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  42. bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

    Plasmid
    #177233
    Purpose
    Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase
    Depositor
    Insert
    sgNeo1/sgPrkaa1/sgPrkaa2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  43. Scramble_gRNA_puro

    Plasmid
    #234999
    Purpose
    scramble sgRNA
    Insert
    scramble sgRNA
    Use
    Lentiviral
    Tags
    EGFP:T2A:Puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 9, 2025
    Availability
    Academic Institutions and Nonprofits only
  44. pML107-RER2

    Plasmid
    #232884
    Purpose
    Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.
    Insert
    RER2 gRNA (RER2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  45. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  46. pNSU299_CPR1_2

    Plasmid
    #166071
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.
    Depositor
    Insert
    Intergenic region near CPR1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  47. pNSU299_GLK1

    Plasmid
    #166090
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    GLK1 (GLK1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  48. pNSU299_PPE1

    Plasmid
    #166083
    Purpose
    Plasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Insert
    PPE1 (PPE1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 1551 - 1600 of 4232 results