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Showing: 621 - 640 of 4183 results

  1. pFsynW SYT1deltaC2AB reporter

    Plasmid
    #197279
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197279-plasmid-map-sequence-id-389097

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  8. pEND-349_Cacnes_ori

    Plasmid
    #225604
    Purpose
    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225604-plasmid-map-sequence-id-451376

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  10. pEND-400_Cacnes_CAMP2

    Plasmid
    #225606
    Purpose
    Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225606-plasmid-map-sequence-id-451402

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  11. pEND-418_Cacnes_LysA

    Plasmid
    #225607
    Purpose
    Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225607-plasmid-map-sequence-id-451378

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  13. pCRISPR-cBEST

    Plasmid
    #125689
    Purpose
    C to T base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Insert
    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    ermE*/tipA
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    125689-plasmid-map-sequence-id-271202

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  20. pEND-193_Cacnes_HS

    Plasmid
    #225622
    Purpose
    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    HspR
    Use
    Synthetic Biology
    Tags
    pFAST-6XHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225622-plasmid-map-sequence-id-451414

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Showing: 621 - 640 of 4183 results