Purpose
Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
Mutation
Changed Pro434 codon from CCG to CCC and Gly468 f…
Available Since
July 23, 2024
Availability
Academic Institutions and Nonprofits only