We narrowed to 945 results for: dcas9, grna

1. pJMP8835

   Plasmid

   #220902

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJMP8823

   Plasmid

   #220901

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJMP8930

   Plasmid

   #220905

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro

   Plasmid

   #174119

   Purpose

   Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)

   Depositor

   Stanley Qi

   Article

   Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

   Insert

   sgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6 and UbC

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJMP8921

   Plasmid

   #220903

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJMP8932

   Plasmid

   #220906

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GB1839

   Plasmid

   #193103

   Purpose

   GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold.

   Depositor

   Diego Orzaez

   Article

   Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

   Insert

   GB_SynP gRNA2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCK411.RR1

   Plasmid

   #192644

   Purpose

   Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   BBa_J23119-RR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

   Plasmid

   #174118

   Purpose

   Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

   Depositor

   Stanley Qi

   Article

   Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

   Insert

   sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6 and UbC

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK434

    Plasmid

    #155378

    Purpose

    PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C114m

    Plasmid

    #121013

    Purpose

    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    dCas9 with no bbsI or BsaI sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pdCRISPomyces-2BD

    Plasmid

    #234429

    Purpose

    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified dCas9(D10A, H840A) with polyaspartate linking
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Promoter

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPRainbow-DONOR1

    Plasmid

    #75398

    Purpose

    CRISPRainbow-DONOR1

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    Cm(R)-CcdB
    

    Use

    CRISPR

    Tags

    no

    Expression

    Mutation

    Promoter

    Bacteria

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBH504

    Plasmid

    #215782

    Purpose

    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene

    Depositor

    Corey Wilson

    Article

    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:

    Inserts

    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bt1311, P(O1), and P1

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK398

    Plasmid

    #155370

    Purpose

    dCas9 sgRNA oscillator v1 (dCRv1) (p15A)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Insert

    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RFN_EGFP_81

    Plasmid

    #60073

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

17. RFN_EGFP_47

    Plasmid

    #60071

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. RFN_EGFP_84

    Plasmid

    #60076

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

19. RFN_EGFP_83

    Plasmid

    #60075

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. RFN_EGFP_82

    Plasmid

    #60074

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only