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pISH-Gucy1b2-probe2

Plasmid

#105455

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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DGP104- pSP64 Poly(A)700

Plasmid

#70098

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

700bp insert from B. subtilis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP105- pSP64 Poly(A)900

Plasmid

#70100

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

900bp insert from B. subtillis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
DGP106- pSP64 Poly(A)1200

Plasmid

#70101

Purpose

an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

Depositor

David Gresham

Article

Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

Insert

1200bp insert from B. subtillis

Use

In vitro transcription

Promoter

SP6

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
SL-TREAT

Plasmid

#196918

Purpose

The TREAT reporter containing cap-proximal GC-rich stemloop

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA

Plasmid

#172911

Purpose

Mammalian expression of voltage sensor

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Ace2N-4AA-mNeon A122D

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

Plasmid

#172912

Purpose

Mammalian expression of voltage sensor; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Insert

Ace2N-4AA-mNeon-ST A122D

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

CAG

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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SL-SunTag

Plasmid

#196919

Purpose

The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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MCV-hVam6p-

Plasmid

#32059

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-hVam6p-

Mutation

TG to GC mutations at 3280-3281 (nt) of full cons…

Promoter

none from vector. Insert contains native promoter…

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pLN34 - pBait-5GC[1xMS2]-McaS

Plasmid

#222403

Purpose

arabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

Depositor

Katherine Berry

Article

Linh D. Nguyen et al Sci Rep 15, 3820 (2025)

Insert

McaS (mcaS E. coli)

Tags

1xMS2 hairpin, 5GC clamp

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-Volton2-ST-WPRE

Plasmid

#172907

Purpose

AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Has Service

AAV1

Insert

Voltron2-ST

Use

AAV and Cre/Lox

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

hSynapsin1

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

Plasmid

#172908

Purpose

AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

Depositor

GENIE Project

Article

Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

Has Service

AAV1

Insert

Ace2N-4AA-mNeon-ST A122D

Use

AAV and Cre/Lox

Tags

soma localization targeting sequence

Expression

Mammalian

Mutation

A122D

Promoter

hSynapsin1

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBBR15.2-2H12.D11opt

Plasmid

#221185

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2.1

Expression

Bacterial

Promoter

Constitutive

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ167

Plasmid

#69309

Purpose

Gateway entry vector with Cas9p

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

Cas9p (Plant codon-optimized; high GC content at 5 prime region)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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GalT-moxDendra2

Plasmid

#89789

Purpose

mammalian expression of GC localized moxDendra2

Depositor

Erik Snapp

Article

Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

Insert

GalT signal anchor transmembrane domain (B4GALT1 Human)

Tags

moxDendra2

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf7

Plasmid

#163597

Purpose

Expresses GDF-7 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf7 (Gdf7 Mouse)

Expression

Mammalian

Mutation

Some Guanin and Cytosine were substituted to redu…

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRAN334

Plasmid

#120814

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Gucy1b2-IRES-tauGFP-ACNF TV

Plasmid

#105211

Purpose

targeting vector to generate a Gucy1b2-IRES-tauGFP mouse strain.

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b2-IRES-tauGFP-ACNF (Gucy1b2 Mouse)

Use

Mouse Targeting

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only
pISH-Gucy1b2-B

Plasmid

#105457

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Bleymehl et al Neuron. 2016 Dec 21;92(6):1196-1203. doi

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy1b3

Plasmid

#105458

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b3 (Gucy1b1 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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