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pJUMP19-L3S1P51_T

Plasmid

#127037

Purpose

Basic Part T- Terminator; L3S1P51; synthetic terminator

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P51_T

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pbad_P

Plasmid

#127003

Purpose

Basic Part P- Promoter (non-constitutive); Pbad (I13453), arabinose induced; controlled by AraC.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Pbad

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-T7pro_P

Plasmid

#127002

Purpose

Basic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part T7 promoter_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Streptag_N

Plasmid

#127034

Purpose

Basic Part N- N-terminus; Strep-tag II

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_N

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1-mutant Fzd3 3'UTR

Plasmid

#25023

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mutant Fzd3 3'UTR

Use

Luciferase

Expression

Mammalian

Mutation

miR-31 binding site in 3’ UTR mutagenized from TC…

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only
pJUMP47ts-2A(sfGFP)

Plasmid

#126992

Purpose

Level 2 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP19-L3S1P51_CT

Plasmid

#127047

Purpose

Basic Part CT- C-terminus + Terminator; L3S1P51. C-termini STOP + terminator (synthetic)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part L3S1P51_CT

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP18-mCherry_O

Plasmid

#127025

Purpose

Basic Part O- ORF; mCherry; fluorescent protein

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part mCherry_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23107_P

Plasmid

#127000

Purpose

Basic Part P- Promoter (constitutive); J23107.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23107_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP19-J23100_P

Plasmid

#126996

Purpose

Basic Part P- Promoter (constitutive); J23100

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23100_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS1-miR-126 site

Plasmid

#25026

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-126 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 (1-428)

Plasmid

#60395

Purpose

Expression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

deleted 429 - end

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinA HDEL

Plasmid

#32117

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

HDEL and Kar2

Expression

Yeast

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE HDEL

Plasmid

#32118

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

HDEL and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Available Since

Oct. 12, 2011

Availability

Academic Institutions and Nonprofits only
pPS1597

Plasmid

#8920

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

TIF51A (HYP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
U371-GFP ILtoEK

Plasmid

#24472

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

GFP

Expression

Bacterial

Mutation

Contains only the kinesin's motor head fused…

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pUdO3z

Plasmid

#210252

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUdO3t

Plasmid

#210248

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUdO3te

Plasmid

#210244

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdO3k

Plasmid

#210236

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge