We narrowed to 10,408 results for: plasmids 101

1. pcDNA3.1-mas-KB-1753-Nluc I9A/W10A

   Plasmid

   #158604

   Purpose

   Plasmid expressing KB-1753-Nluc I9A/W10A in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 I9A/W10A - linker (GGTGGS) - Nluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1-mas-GRK2RH-Nluc L118A

   Plasmid

   #158606

   Purpose

   Plasmid expressing GRK2(RH)-Nluc L118A in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain L118A - linker (GGTGGS) - Nluc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGL2-MCT2Prom0.6

   Plasmid

   #87353

   Purpose

   Human monocarboxylate transporter 2 (MCT2) promoter 0.6 kbp

   Depositor

   Saroj Mathupala

   Article

   Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

   Insert

   MCT2 (SLC16A7 Human)
   

   Use

   Luciferase

   Expression

   Bacterial and Mammalian

   Promoter

   0.6 kbp proximal promoter of human MCT2 (nested d…

   Available Since

   March 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. psiCheck-DNAJA4CDS

   Plasmid

   #41849

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

   Insert

   DNAJA4 CDS (DNAJA4 Human)
   

   Use

   Luciferase

   Tags

   renilla luciferase

   Mutation

   Start codon of DNAJA4 removed

   Available Since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pAT-9222

   Plasmid

   #124222

   Purpose

   Cloning plasmid for EGxFP assay with self-targeting gRNA-s

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   self-targeting gRNA between EGFP halves
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJS278

   Plasmid

   #134139

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-Precission-human Ska1 MTBD (SKA1 Human)
   

   Expression

   Bacterial

   Mutation

   aa 132-255, R155A

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

   Plasmid

   #127308

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

   Plasmid

   #127316

   Purpose

   Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG and CL1/PEST

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

   Plasmid

   #124859

   Purpose

   Mutagenesis of Slc17a7

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a7 (Slc17a7 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127318

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pClneoFH-let60 G12V

    Plasmid

    #66860

    Purpose

    Express FLAG-His6-tagged dominant active let60, in which Glycine 12 is mutated to Valine, in mammalian cells

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

    Insert

    Let-60 (let-60 Nematode)
    

    Tags

    FLAG-His6

    Expression

    Mammalian

    Mutation

    Glycine 12 is mutated to valine

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRS316-VTC4

    Plasmid

    #108691

    Purpose

    VTC4 sequence expressed in yeast plasmid

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Vacuolar Transporter Chaperone 4
    

    Expression

    Yeast

    Promoter

    VTC4 (native promoter)

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCheck-ApoE3'UTR

    Plasmid

    #41846

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    ApoE 3'UTR (APOE Human)
    

    Use

    Luciferase

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pJ02B2Gm:Rm_AF

    Plasmid

    #66061

    Purpose

    MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJ02B2Rm:Gm_AF

    Plasmid

    #66062

    Purpose

    MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. t2. pT7-7-SNCA-5Lys

    Plasmid

    #107294

    Purpose

    Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-5Lys (SNCA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. r. pT7-7-SNCA-5Asn

    Plasmid

    #107295

    Purpose

    Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-5Asn (SNCA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. s2. pT7-7-SNCA-5Asp

    Plasmid

    #107293

    Purpose

    Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-5Asp (SNCA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. gh173

    Plasmid

    #106834

    Purpose

    expression of gRNA targeting UGGT2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    UGGT2 (UGGT2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSR04

    Plasmid

    #69151

    Purpose

    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    eGFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    eef-1A.1 (eft-3) and rps-27

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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