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pRK5-FLAG-Sestrin2-S190W

Plasmid

#73670

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S190W changed serine 190 to tryptophan

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pXS-dTomato

Plasmid

#117387

Purpose

pGEN-mcs with a modular dTomato expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

dTomato

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-Sestrin2-E451A

Plasmid

#73677

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax(TadA, TadA*E59A)

Plasmid

#125662

Purpose

pCMV-ABEmax(TadA, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMN-326

Plasmid

#195451

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF3 BS - minTK - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-177

Plasmid

#195425

Purpose

Transient, constitutive expression of synZiFTR: ZF2-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF2-6 (ZF2) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-179

Plasmid

#195427

Purpose

Transient, constitutive expression of synZiFTR: ZF4-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF4-8 (ZF4) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-182

Plasmid

#195430

Purpose

Transient, constitutive expression of synZiFTR: ZF7-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF7-3 (ZF7) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-183

Plasmid

#195431

Purpose

Transient, constitutive expression of synZiFTR: ZF8-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF8-1 (ZF8) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-184

Plasmid

#195432

Purpose

Transient, constitutive expression of synZiFTR: ZF9-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF9-2 (ZF9) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-186

Plasmid

#195434

Purpose

Transient, constitutive expression of synZiFTR: ZF11-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pUb - 3XFLAG - NLS - ZF11-1 (ZF11) - p65 - RbGpA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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IFP1.4-C1

Plasmid

#54782

Purpose

Localization: C1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Roger Tsien

Article

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Type

Empty backbone

Tags

IFP1.4

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
sv272-HisMBP-TZAPZNF9-11

Plasmid

#87185

Purpose

bacterial expression of HisMBP-TZAPZNF9-11

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Tags

HisMBP

Expression

Bacterial

Promoter

T7

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAct-dCBEevoAPOBEC1

Plasmid

#195514

Purpose

Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

dCBE-evoAPOBEC1

Use

CRISPR

Expression

Insect

Promoter

act5C

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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p416TEF-SEC16

Plasmid

#102676

Purpose

The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

Depositor

Jens Nielsen

Article

Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

Insert

SEC16 (SEC16 Budding Yeast)

Expression

Yeast

Promoter

TEF

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfCFPBb

Plasmid

#118232

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfCFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfCFP

Expression

Bacterial

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Mis12 INCENP Δcen mCherry

Plasmid

#45228

Depositor

Michael Lampson, Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12 INCENP Δcen mCherry

Tags

VSVG

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only
PacrAB-rfp + PmutS-yfp

Plasmid

#121442

Purpose

plasmid reporting the expression from the acrAB and mutS promoters

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-yfp
PacrAB-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

PacrAB and PmutS

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEBG-GST-TRAF2

Plasmid

#44101

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
AP-VAMP1

Plasmid

#167996

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP1(T29-K89)-T7PAP

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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