We narrowed to 11,105 results for: HA

Showing: 6401 - 6420 of 11105 results

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plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA

Plasmid

#218527

Purpose

sgRNA targeting human EIF2AK2/PKR

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK2 (EIF2AK2 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-FLEX-macaque OPN4-IRES-EGFP

Plasmid

#223253

Purpose

AAV construct for Cre-dependent (FLEX) expression of macaque melanopsin (Opn4, short isoform, GenBank:OP856641; hSyn promoter) and of EGFP via an IRES.

Depositor

Michael Do

Article

Liu et al Science. 2023 Jan 27;379(6630):376-381.

Insert

macaque OPN4-IRES-EGFP (OPN4 )

Use

AAV

Promoter

human Synapsin 1 (hSyn)

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-Sec61b-C1

Plasmid

#90993

Purpose

Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mApple-Sec61b (SEC61B Human, Synthetic)

Tags

mApple (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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DDX5

Plasmid

#155523

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX5 (DDX5 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-HSV TK-P2A-H2-Kb SCT-T2A-mCD47-E2A-mCD55-F2A-neo-AAVS1

Plasmid

#205446

Purpose

human AAVS1-targeting plasmid for expression of HSV TK, H2-Kb SCT, mouse CD47, and mouse CD55

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HSV TK-H2-Kb SCT-mCD47-mCD55

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_Zeo_TetO_FLAG-AT1R

Plasmid

#222856

Purpose

Mammalian expression plasmid for FLAG-tagged AT1R

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT1R (AGTR1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B

Plasmid

#106309

Purpose

The plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.

Depositor

James Zhe Liu

Article

Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

Insert

6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)

Tags

6xflag-3xHaloTag(MtoL)

Expression

Mammalian

Promoter

EF1

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Lysosome-N-20

Plasmid

#231778

Purpose

Visualization of lysosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Lysosome-N-20 (Lamp1 Rat)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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TARDBP

Plasmid

#156073

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TARDBP (TARDBP Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B EDD

Plasmid

#37188

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

CMV

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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AFDN_PDZ_1

Plasmid

#103938

Purpose

Protein expression and purification of TRITHORAX PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

AFDN (AFDN Human)

Tags

GST

Expression

Bacterial

Mutation

contains AA983-1110 of AFDN

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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CLK1

Plasmid

#155458

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CLK1 (CLK1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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DLG1_PDZ_3

Plasmid

#103917

Purpose

Protein expression and purification of DLG1 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG1 (DLG1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-GPAT4152-208

Plasmid

#134468

Purpose

GPAT4152-208 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

GPAT4 (GPAT4 Human)

Expression

Mammalian

Mutation

GPAT4, amino acids 152-208

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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DLG1_PDZ_2

Plasmid

#103916

Purpose

Protein expression and purification of DLG1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG1 (DLG1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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DLG1_PDZ_1

Plasmid

#103915

Purpose

Protein expression and purification of DLG1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG1 (DLG1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Tubulin-C-18

Plasmid

#231779

Purpose

Visualization of tubulin in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Tubulin-C-18 (TUBA1B Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-Flpo

Plasmid

#174378

Purpose

also known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Has Service

AAV Retrograde

Insert

FLPo recombinase

Use

AAV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC592 - pCMV Mito-iRFP

Plasmid

#59135

Purpose

A plasmid that express mitochondrial-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Mitochondria-localized Infrared Fluorescent Protein

Tags

Mitochondria-targeting sequence

Expression

Mammalian

Promoter

CMV-IE

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P2(GST-SNAP-Shp2tSH2)

Plasmid

#177907

Purpose

Baculo expression of GST tag fused to SNAPtag and human Shp2 tandem SH2 domains

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

Shp2 tandem SH2 domains (aa 6-218 only) (PTPN11 Human)

Tags

GST-PreScission cut site-SNAP tag

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2025

Availability

Academic Institutions and Nonprofits only

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