We narrowed to 12,187 results for: LEA
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Plasmid#179691PurposeBacterial expression of base editorDepositorInsert[sd4U] UGI-TALE3-DddA-G1397-N (Q1310R + S1330I + T1380I)
ExpressionBacterialMutationQ1310R, S1330I, T1380IAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
sTR056
Plasmid#177369PurposeToehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26
Plasmid#149346PurposeMammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNADepositorInsertCas9
UseCRISPRTagsFlag tagExpressionMammalianPromoterCbcAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Venus-pA_U6-BbsI (pE057)
Plasmid#149351PurposeMammalian expression vector for VenusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVenus
ExpressionMammalianPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BFP-pA_U6-BbsI (pE058)
Plasmid#149352PurposeMammalian expression vector for BFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBFP
ExpressionMammalianPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-miRFP670-pA_U6-BbsI (pE059)
Plasmid#149353PurposeMammalian expression vector for miRFP670DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmiRFP670
ExpressionMammalianPromoterCAGAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
shFGF2#2
Plasmid#157668PurposeTet inducible knockdown of FGF2DepositorAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
wt Cus2p
Plasmid#73046PurposeWild-type S. cerevisiae Cus2p gene for recombinant expression and crystallizationDepositorPublicationInsertCUS2p (CUS2 Budding Yeast)
Tags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastPromoterT7Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBHSTC97
Plasmid#124352PurposePlasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxinDepositorInsertNpuDnaBΔ283 mini-intein
TagsCcdA-C (SFAD)ExpressionBacterialMutationDeletion of 283-residue endonuclease domainPromoterSTCAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC81
Plasmid#118900Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GSTDepositorInsertNcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease; GST tagExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC219
Plasmid#118908Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tagDepositorInsertNcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
TagsYBBR tag, HIS10, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0011
Plasmid#117530PurposeLevel0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)DepositorInsertSp-xCas9 3.7 (no stop codon)
UseCRISPRMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5A1-Ti
Plasmid#92018PurposeExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV proteaseDepositorInsertsTALE targeting lac operator with 3 TEV cleavage sites
Catalytically Inactive TEV Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219V, C151AAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sph1-VC
Plasmid#89756Purposeexpresses human Synphilin fused to the C-terminal part of VenusDepositorAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB775
Plasmid#60115PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)DepositorInsertYeLuc
UseUnspecifiedTagsClpXP degron and Nuclear localization signal (NLS…Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB776
Plasmid#60117PurposeRed beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)DepositorInsertRdLuc
UseUnspecifiedTagsClpXP degron and Nuclear localization signal (NLS…Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
cx52.9_R (OZ584)
Plasmid#35224DepositorInsertZinc finger array targeting cx52.9 (cx52.9 Zebrafish)
UseZebrafish targetingAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
cx52.9_L (OZ583)
Plasmid#35223DepositorInsertZinc finger array targeting cx52.9 (cx52.9 Zebrafish)
UseZebrafish targetingAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
panx2_R (OZ602)
Plasmid#35242DepositorInsertZinc finger array targeting panx2 (panx2 Zebrafish)
UseZebrafish targetingAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
panx2_L (OZ601)
Plasmid#35241DepositorInsertZinc finger array targeting panx2 (panx2 Zebrafish)
UseZebrafish targetingAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only