We narrowed to 15,723 results for: LEA

1. pGAPDH::H2B-mScarlet

   Plasmid

   #186764

   Purpose

   A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   H2B-mScarlet
   

   Use

   Overexpression

   Tags

   H2B

   Promoter

   GAPDH

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENeGFP PCNAL2 pc653

   Plasmid

   #167564

   Purpose

   The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

   Depositor

   Cristina Cardoso

   Article

   Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

   Insert

   Proliferating Cell Nuclear Antigen (PCNA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVC299 VEGF Site#2

   Plasmid

   #47506

   Purpose

   human gRNA expression vector targeting VEGF

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-VEGF site 2 (VEGFA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-CD81-(TEV)-FKBP-Myc

   Plasmid

   #177908

   Purpose

   human CD81-TEV cleavage site-FKBP-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   human CD81 (CD81 Human, Synthetic)
   

   Tags

   FKBP, Myc tag, and TEV protease cleavage site

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC446 - pAAV EF1a hPREP(S554A)

   Plasmid

   #59968

   Purpose

   An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S554A

   Promoter

   EF1a

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL11055

   Plasmid

   #68252

   Purpose

   Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICE-HA-CtIP-siR-WT

   Plasmid

   #82030

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   CtIP (RBBP8 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mutations making the cDNA resistant to a siRNA

   Promoter

   CMV-tet

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pClneoMyc human MST2

   Plasmid

   #37022

   Depositor

   Yutaka Hata

   Article

   Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

   Insert

   MST2 (STK3 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pLX_317-C7orf26v1

   Plasmid

   #178537

   Purpose

   Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_317 backbone

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Insert

   C7orf26v1 (INTS15 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-KLF3

    Plasmid

    #49100

    Purpose

    Expresses KLF3 in mammalian cells

    Depositor

    Merlin Crossley

    Article

    Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

    Insert

    Krüppel-Like Factor 3(KLF3)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pEmpty

    Plasmid

    #214051

    Purpose

    Empty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas system

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-DIO-(STChRger2-TS-EYFP)

    Plasmid

    #129394

    Purpose

    Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    soma targeted ChRger2
    

    Use

    AAV and Cre/Lox

    Tags

    Kv2.1-TS-EYFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNS19-LV-mutRFP-2A-GFP

    Plasmid

    #113716

    Purpose

    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    mutRFP-2A-GFP-IRES-Puro
    

    Use

    Lentiviral; Reporter system

    Expression

    Mammalian

    Mutation

    Y64L (mutRFP)

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-GRAB_r5-HT1.0

    Plasmid

    #208725

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

    Plasmid

    #176839

    Purpose

    To recruit SAM complex to human CYP3A4 proximal enhancer.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer sgRNA for activation
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-Flag-NEDD4L∆WW1,2,4

    Plasmid

    #171137

    Purpose

    expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L (NEDD4L Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 221 to alanine, tryptophan 453…

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. anxA10-GFP

    Plasmid

    #107199

    Purpose

    Expresses human annexin A10-GFP fusion protein for live cell imaging

    Depositor

    Volker Gerke, Ursula Rescher

    Article

    Quiskamp et al Cell Mol Life Sci. 2014 Jan;71(2):311-29

    Insert

    annexin A10 (ANXA10 Human)
    

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E_BAG3P209L-eGFP

    Plasmid

    #98181

    Purpose

    Codes for full length, GFP tagged mutant Bag3

    Depositor

    Robert Bryson-Richardson

    Article

    Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

    Insert

    BAG3P209L-eGFP (BAG3 Human)
    

    Use

    Gateway vector

    Tags

    GFP

    Mutation

    P209L

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. 2xFKBP-smFP(myc)-KDEL

    Plasmid

    #173021

    Purpose

    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    2xFKBP-smFP(myc)-KDEL
    

    Tags

    FKBP and smFP(myc) non-fluorescent

    Expression

    Mammalian

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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