We narrowed to 15,599 results for: LEA

1. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75232

   Purpose

   CRISPR/Cas9 plasmid against human NFATc2

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hy_pMT Flag-Hel25E K91N

   Plasmid

   #110121

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   K91N mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hy_pMT Flag-Hel25E D193E

   Plasmid

   #110120

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   D193E mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

   Plasmid

   #127865

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Expression

   Mutation

   deleted the c-terminal 20 amino acids

   Promoter

   hSyn

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. ADGRL3-CTF-Flag

   Plasmid

   #201219

   Purpose

   pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

   Plasmid

   #166413

   Purpose

   express hPOT1 ΔOB in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   Protection Of Telomeres 1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   ΔOB deleted 125 a.a

   Promoter

   ef1-a

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

   Plasmid

   #210504

   Purpose

   expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pClneoMyc MOB1 T12/35A

   Plasmid

   #47938

   Purpose

   expresses Myc tagged human MOB1 containing T12A and T35A mutations

   Depositor

   Yutaka Hata

   Article

   Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

   Insert

   MOB1A (MOB1A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T12A and T35A

   Promoter

   CMV

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

9. Polymerase expression - pCMV-B103 (LM2907)

   Plasmid

   #208968

   Purpose

   Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   B103-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. DICER KO vector

    Plasmid

    #16656

    Depositor

    Bert Vogelstein

    Article

    Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

    Insert

    Dicer knock-out (DICER1 Human)
    

    Use

    Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

11. mCHOP10(dLZ).pCDNA1

    Plasmid

    #21900

    Depositor

    David Ron

    Article

    Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

    Insert

    CHOP10 (Ddit3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The coding region of the murine CHOP-10 cDNA was …

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

    Plasmid

    #44597

    Depositor

    Susan Semple-Rowland

    Article

    Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

    Insert

    EF1a-GFP:2A:CHER(HA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    human elongation factor -1a (pos 2528-3971)

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid

    #85820

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc Dam_f_GFPoNLS
    

    Use

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A.

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

    Plasmid

    #127866

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Expression

    Mutation

    C-terminal 20 amino acids only

    Promoter

    hSyn

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWay21-MKP-1 ASA

    Plasmid

    #13479

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1ASA (Dusp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Arginine 53 to Alanine, Changed Arginine…

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

16. p3E-DrCav1

    Plasmid

    #194290

    Purpose

    Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    caveolin1 (cav1.S Frog)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-DNPH1

    Plasmid

    #203224

    Purpose

    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    DNPH1 (DNPH1 Human)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MDH1-miR-31SM-PGK-GFP-Blast

    Plasmid

    #31516

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-31 (Mir31 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Promoter

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pBABEpuro-Flag-YAP2

    Plasmid

    #27472

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

20. Nup98-pDONR221

    Plasmid

    #124241

    Purpose

    Gateway entry clone of Drosophila Nup98-96 cDNA.

    Depositor

    Peter Vilmos

    Article

    Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

    Insert

    Drosophila Nup98-96 (Nup98-96 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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