We narrowed to 10,413 results for: plasmids 101

1. pAAV-hSyn-DIO-NES-jRGECO1a-WPRE

   Plasmid

   #216276

   Purpose

   AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1a

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   NES-jRGECO1a
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Promoter

   human synapsin 1 (hSyn)

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Ef1a-FAS-GCaMP6f-WPRE

   Plasmid

   #141237

   Purpose

   AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Promoter

   Human elongation factor-1 alpha (EF-1 alpha)

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

   Plasmid

   #174897

   Purpose

   ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sf GFP
   

   Expression

   Mammalian

   Mutation

   150TAA in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

   Plasmid

   #174898

   Purpose

   opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TGA in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHR-LS

   Plasmid

   #205438

   Purpose

   Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes

   Depositor

   Masamichi Kamihira

   Article

   Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

   Inserts

   Gaussia luciferase gene
   spectinomycin resistance gene
   

   Use

   Cre/Lox and Luciferase; Chlamydomonas expression

   Promoter

   Heat shock protein 70A (HSP70A)/ribulose bisphosp…

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBABR mCherry-Bcd-LEXY

   Plasmid

   #182596

   Purpose

   Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

   Depositor

   Jared Toettcher

   Article

   Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

   Insert

   Bcd (bcd Fly)
   

   Tags

   LEXY and mCherry

   Expression

   Insect

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB-TAC-OKMS-KRAB-dCas9

   Plasmid

   #120356

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GSX2-P2A-tGFP-DTA

   Plasmid

   #161750

   Purpose

   tGFP plasmid for the c-terminal targeting of human GSX2 locus

   Depositor

   Elena Cattaneo

   Article

   Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

   Insert

   turboGFP
   

   Use

   CRISPR

   Tags

   P2A-tGFP

   Expression

   Mammalian

   Promoter

   endogenous GSX2 promoter

   Available Since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIW040

   Plasmid

   #134127

   Purpose

   Bacterial expression of human Ska1 microtubule binding domain (MTBD)

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-Precission-human Ska1 MTBD (SKA1 Human)
   

   Expression

   Bacterial

   Mutation

   aa 132-255 R236A

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIW039

    Plasmid

    #134126

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Expression

    Bacterial

    Mutation

    aa 132-255 and K203A K206A

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIW028

    Plasmid

    #134125

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Expression

    Bacterial

    Mutation

    aa 132-255 and K183A K184A

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PLKO.1-UPF3B-3'UTR

    Plasmid

    #136044

    Purpose

    UPF3B shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CAGGGCAAAGAATAGAGAGAA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF3B (UPF3B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPRIME-CMV-GFP-shNts2

    Plasmid

    #132714

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(2) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPRIME-CMV-GFP-shNts1

    Plasmid

    #132713

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(1) (Nts Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

    Plasmid

    #127335

    Purpose

    Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    Firefly luciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG

    Plasmid

    #127309

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127317

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST

    Plasmid

    #127313

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127319

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG

    Plasmid

    #127311

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge