We narrowed to 13,804 results for: TIM

1. CMV_R-PTEN

   Plasmid

   #227433

   Purpose

   Expresses the R-PTEN sensor under a CMV promoter

   Depositor

   Tal Laviv

   Article

   Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

   Insert

   R-PTEN (Pten Rat)
   

   Use

   Tags

   mCyRFP2, mMaroon

   Expression

   Mammalian

   Mutation

   R14G

   Promoter

   CMV

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV_G-PTEN_4A

   Plasmid

   #227434

   Purpose

   Expresses the G-PTEN sensor with a 4A mutation

   Depositor

   Tal Laviv

   Article

   Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

   Insert

   G-PTEN 4A (Pten Rat)
   

   Use

   Tags

   mEGFP and sREACh

   Expression

   Mammalian

   Mutation

   4A mutation.

   Promoter

   CMV

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCpep-E1GFP

   Plasmid

   #233884

   Purpose

   Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

   Depositor

   Francesco Cardarelli

   Article

   De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

   Insert

   Proinsulin-E1GFP (Ins2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Inserted E1GFP into C-peptide of mouse Ins2 codin…

   Promoter

   CMVF

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 IST1 L328D, L355A (1-366)

   Plasmid

   #193040

   Purpose

   Bacterial expression plasmid for full-length IST1 (1-366) with L328D and L355A mutations that diminish binding to MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L328D, L355A

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG CAPN7 V18D-OSF

   Plasmid

   #193042

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   V18D

   Promoter

   Chicken Beta-actin

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG CAPN7 L61D-OSF

   Plasmid

   #193043

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   L61D

   Promoter

   Chicken Beta-actin

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG CAPN7 F98D-OSF

   Plasmid

   #193044

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   F98D

   Promoter

   Chicken Beta-actin

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-CAPN7-V18D-mCherry

   Plasmid

   #193046

   Purpose

   Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   V18D, siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Tight TRE

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-CAPN7-C290S-mCherry

   Plasmid

   #193047

   Purpose

   Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C290S, siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Tight TRE

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 CAPN7 V18D,F98D (1-165)

    Plasmid

    #193034

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    V18D, F98D, Residues 1-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PD2529 human beta1 ecto

    Plasmid

    #221398

    Purpose

    Mammalian expression of human integrin beta1 ectodomain

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    integrin beta1 ectodomain (ITGB1 Human)
    

    Use

    Tags

    AVI tag, HRV3C cleavage site, basic coil, HA tag,…

    Expression

    Mammalian

    Mutation

    codon optimized for human mature _1 residues Q1 t…

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-CLIP-CD28(CD45TM)

    Plasmid

    #223629

    Purpose

    Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Expression

    Mutation

    CD28 transmembrane domain replaced by CD45 transm…

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-Spy-CD28(CD45TM)

    Plasmid

    #223624

    Purpose

    Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Spytag003

    Expression

    Mutation

    CD28 transmembrane domain replaced by CD45 transm…

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-SNAP-CD86(F56A)

    Plasmid

    #223609

    Purpose

    Lentiviral expression of human CD86 (F56A) with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    F56A

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-SNAP-CD80(Y65A)

    Plasmid

    #223608

    Purpose

    Lentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    SNAP

    Expression

    Mutation

    Y65A

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-CLIP-CD28(SGGG)

    Plasmid

    #223607

    Purpose

    Lentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Expression

    Mutation

    MYPPPY mutated to SGGG

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPPI4-Strep-SNAP-CD28-GCN4-His6

    Plasmid

    #223585

    Purpose

    Protein purification of human CD28 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD28 (CD28 Human)
    

    Use

    Tags

    GCN4, His6 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPPI4-Strep-SNAP-CTLA-4-GCN4-His6

    Plasmid

    #223582

    Purpose

    Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Tags

    GCN4, His6 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPPI4-Strep-SNAP-CD80 (I92R)-His10

    Plasmid

    #223580

    Purpose

    Protein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Tags

    His10 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    I92R

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPPI4-Strep-SNAP-CD80-His10

    Plasmid

    #223579

    Purpose

    Protein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Tags

    His10 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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