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We narrowed to 8,284 results for: crispr grnas

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Showing: 6441 - 6460 of 8284 results

  4. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  5. pLKO H2BmRFP-2A-puro HA v4 trcr filler

    Plasmid
    #256785
    Purpose
    lentiviral construct with H2BmRFP-2A-puromycin, HA, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
    Depositor
    Daniel Schramek
    Insert
    H2B-mRFP (H2B Human, Synthetic)
    Use
    Lentiviral
    Tags
    HA
    Available Since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    256785-plasmid-map-sequence-id-513553

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  6. pLKO H2BmRFP-2A-PLGRKT v4 trcr filler

    Plasmid
    #256781
    Purpose
    lentiviral construct with H2BmRFP-2A-PLGRKT (human) and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
    Depositor
    Daniel Schramek
    Insert
    PLGRKT (PLGRKT Human)
    Use
    Lentiviral
    Available Since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    256781-plasmid-map-sequence-id-513547

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  7. pLKO H2BmRFP-2A-Plgrkt v4 trcr filler

    Plasmid
    #256782
    Purpose
    lentiviral construct with H2BmRFP-2A-Plgrkt (mouse) and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
    Depositor
    Daniel Schramek
    Insert
    Plgrkt (Plgrkt Mouse)
    Use
    Lentiviral
    Available Since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    256782-plasmid-map-sequence-id-513546

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  8. pLKO H2BmRFP-2A-puro PLGRKT v4 trcr filler

    Plasmid
    #256788
    Purpose
    lentiviral construct with H2BmRFP-2A-puromycin, PLGRKT, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
    Depositor
    Daniel Schramek
    Insert
    PLGRKT (PLGRKT Human)
    Use
    Lentiviral
    Available Since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    256788-plasmid-map-sequence-id-513542

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  9. pLKO H2BmRFP-2A-puro Plgrkt v4 trcr filler

    Plasmid
    #256786
    Purpose
    lentiviral construct with H2BmRFP-2A-puromycin, Plgrkt, and U6 driven gRNA cassette with the v4 modified trcrRNA. gRNAs can be cloned in by digesting the filler with BsmBI.
    Depositor
    Daniel Schramek
    Insert
    Plgrkt (Plgrkt Mouse)
    Use
    Lentiviral
    Available Since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    256786-plasmid-map-sequence-id-513535

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  12. pGGA-FT guide RNA1_7

    Plasmid
    #246797
    Purpose
    A GreenGate entry vector containing a guide RNA expression cassette targeting the AtFT gene with 7 different gRNAs
    Depositor
    Danhua Jiang
    Insert
    AtU6-1 promoter-FT guide RNA1-AtU6-1 promoter-FT guide RNA3- (FT Mustard Weed, Synthetic)
    Use
    CRISPR; Greengate cloning entry vector
    Expression
    Plant
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    246797-plasmid-map-sequence-id-495594

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  13. pSpec5'

    Plasmid
    #228549
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228549-plasmid-map-sequence-id-454858

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  14. pSpec3'

    Plasmid
    #228548
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228548-plasmid-map-sequence-id-454857

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  15. pX330-U6-MYC-Guide-hSpCas9

    Plasmid
    #228574
    Purpose
    Expression of human MYC gRNA; CRISPR mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    MYC gRNA (MYC Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228574-plasmid-map-sequence-id-457356

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  18. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  19. pPbU6_hdhfr/yfcu_Cas9

    Plasmid
    #216423
    Purpose
    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.
    Depositor
    Ellen Bushell
    Article
    Jonsdottir et al bioRxiv 2024.04.20.590404
    Type
    Empty backbone
    Use
    CRISPR; Expression in plasmodium berghei
    Expression
    Bacterial
    Available Since
    April 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    216423-plasmid-map-sequence-id-431289

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  20. tRNA-gRNA position [M1_n-1] (GB1210)

    Plasmid
    #75411
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_n-1]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75411-plasmid-map-sequence-id-211947

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Showing: 6441 - 6460 of 8284 results