We narrowed to 14,580 results for: pET

1. pET28a-LSSmGFP-TEV-mScarlet-i

   Plasmid

   #191199

   Purpose

   Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & mScarlet-I fusion
   

   Use

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-LSSmGFP-TEV-SAV

   Plasmid

   #191200

   Purpose

   Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET22b-T5-MfnG-TEV-His

   Plasmid

   #191114

   Purpose

   Expresses MfnG-TEV-His in E. coli

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   MfnG
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-PKP3(305-797)

   Plasmid

   #202599

   Purpose

   Expresses ARM domain of plakophilin3

   Depositor

   Tina Izard

   Article

   Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

   Insert

   plakophilin3 (PKP3 Human)
   

   Use

   Tags

   His8 tag with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1 to 304

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

   Plasmid

   #187708

   Purpose

   Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 381-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-380

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)-NEDD4L_W3-W4-HECT (492)

   Plasmid

   #187709

   Purpose

   Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-491

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-a(+)-6xHis-miRFP(-5)-HO1

   Plasmid

   #219424

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Inserts

   miRFP682
   HO1
   

   Use

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   Contains Ribosomal Binding Site for expression of…

   Promoter

   T7

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-NPR4 (SA binding domain)

   Plasmid

   #226885

   Purpose

   Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form

   Depositor

   Ning Zheng

   Article

   Wang et al Nature. 2020 Oct;586(7828):311-316. doi:

   Insert

   The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   NPR4 SA-binding core (residues 373-516), with a s…

   Promoter

   T7

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a HsDHFR-bio-10His

   Plasmid

   #193883

   Purpose

   Expresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequence

   Depositor

   James Inglese

   Article

   Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

   Insert

   human dihydrofolate reductase (DHFR) (DHFR Human)
   

   Use

   Tags

   His tag and biotinylation sequence

   Expression

   Bacterial

   Mutation

   Codon optimized

   Promoter

   T7

   Available since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-UBE3A iso 2 N terminus

    Plasmid

    #185643

    Purpose

    expression of His-tagged UBE3A isoform 2 1-87 in bacterial cells

    Depositor

    Irina Bezsonova

    Insert

    ubiquitin-protein ligase E3A (UBE3A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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