19,658 results

1. pAAV-hSyn-hChR2(H134R)-mCherry

   Plasmid

   #26976

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV5, AAV8, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   hSyn

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD33-Gm

   Plasmid

   #65098

   Purpose

   bacterial expression vector with Gentamycin selection, based on pBAD33

   Depositor

   Juan Tomas

   Article

   Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tet-inducible mCherry reporter

   Plasmid

   #64128

   Purpose

   Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   mCherry
   

   Expression

   Mammalian

   Promoter

   TetOx13-CMV

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9n(BB) (PX460)

   Plasmid

   #48873

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SpyCatcher-mi3

   Plasmid

   #112255

   Purpose

   Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   SpyCatcher-mi3
   

   Tags

   C-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-mCherry

   Plasmid

   #197420

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FLAG-HA-hYTHDC1

   Plasmid

   #85167

   Purpose

   Expression of FLAG-HA-tagged human YTHDC1

   Depositor

   Samie Jaffrey

   Article

   Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Tags

   Flag and Ha

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL410_INS421

   Plasmid

   #49057

   Purpose

   Expresses luciferase under control of human insulin gene promoter

   Depositor

   Kevin Ferreri

   Article

   Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

   Insert

   human INS promoter (INS Human)
   

   Expression

   Mammalian

   Promoter

   INS promoter

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTagBFP2-MannII-N-10

   Plasmid

   #55309

   Purpose

   Localization: Mannosidase/Golgi, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   MannII
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   aa 1-112 of MannII

   Promoter

   CMV

   Available Since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET-30-His6-SUMO-AEY-CfaN

    Plasmid

    #122949

    Purpose

    Bacterial expression of N-intein

    Depositor

    Thomas Muir

    Article

    Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

    Insert

    CfaN
    

    Expression

    Bacterial

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-flag YAP2 5SA

    Plasmid

    #27371

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP2 (YAP1 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Serines at positions 61, 109, 127, 128, 131, 163,…

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-EFS-MCP-HALOnls

    Plasmid

    #121937

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    MCP-HALOnls
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV5-Flag-HDAC3

    Plasmid

    #63676

    Purpose

    Mammalian expression of Flag tagged HDAC3

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

    Insert

    HDAC3 (Hdac3 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pIRES-HMGA1

    Plasmid

    #13466

    Depositor

    Edward Whang

    Article

    Liau et al Cancer Res. 2006 Dec 15. 66(24):11613-22

    Insert

    HMGA1 (HMGA1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET51b(+)_EGFP_NbALFA

    Plasmid

    #136626

    Purpose

    Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-EGFP-Rac1-Q61L

    Plasmid

    #12981

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lamp1-mTurquoise2

    Plasmid

    #98828

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lamp1
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSP955

    Plasmid

    #98784

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-ENLYFQS-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Str-KDEL_TNF-SBP-EGFP

    Plasmid

    #65278

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVS1

    Plasmid

    #33143

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Insert

    LacO (256 copies)
    

    Expression

    Yeast

    Available Since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only