20,062 results

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pLenti-5HRE-GFP PuroR

Plasmid

#128958

Purpose

Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

5X HRE of VEGF (VEGFA Human)

Use

Lentiviral

Tags

d2EGFP

Expression

Mammalian

Promoter

minimal CMV promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Golgi-7

Plasmid

#58018

Purpose

Localization: Golgi Complex, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Golgi (B4GALT1 Human)

Tags

mTagRFP

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVpuro-TagRFP657

Plasmid

#96939

Purpose

Retroviral plasmid containing TagRFP657 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Tag Red Fluorescent Protein 657

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iTEV

Plasmid

#64277

Purpose

Expresses infrared fluorescent tobacco etch virus protease sensor (iTEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent tobacco etch virus protease sensor (iTEV)

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pMAL-c2X

Plasmid

#75286

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 18, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLX307 MCL1

Plasmid

#117726

Purpose

Open reading frame vector encoding MCL1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

MCL1L (MCL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pIND(V5)EcoDam

Plasmid

#59201

Purpose

Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGKB

Plasmid

#85077

Purpose

expression in Pichia pastoris

Depositor

Sheng Yang

Article

Yang et al Yeast. 2014 Apr;31(4):115-25.

Type

Empty backbone

Expression

Yeast

Promoter

GAP

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TLR targeting vector

Plasmid

#64215

Purpose

Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

traffic light reporter (TLR) (AAVS1 Human)

Use

Human targeting

Mutation

see publication for details

Promoter

CAG

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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p-dCas9-HDAC1-Hygro

Plasmid

#104409

Purpose

transient expression of dCas9-HDAC1 fusion protein

Depositor

Stephan Beck

Insert

HDAC1 (HDAC1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_WT

Plasmid

#81734

Purpose

Gateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

Plasmid

#139987

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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NG-ABEmax

Plasmid

#124163

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

TadA-TadA(evo)-Cas9-NG

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Cre Stoplight 2.4

Plasmid

#37402

Purpose

Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

Depositor

Thomas Hughes

Article

Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

Insert

Cre Stoplight 2.4

Tags

ZsGreen1 and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2_IRES2_EGFP_KCNQ2 var4

Plasmid

#173154

Purpose

For mammalian expression of KCNQ2 and creating variants

Depositor

Al George

Article

Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

Insert

KCNQ2 (KCNQ2 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Promoter

Simian CMV IE94

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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HA-human-BNIP3

Plasmid

#100781

Purpose

Mammalian expression of BNIP3

Depositor

Joseph Gordon

Article

Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

Insert

human-BNIP3 (BNIP3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pPGKPhiC31obpA

Plasmid

#13795

Purpose

Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

Depositor

Philippe Soriano

Article

Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

Insert

PhiC31o

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChrimsonR-GFP

Plasmid

#58806

Purpose

AAV expression of ChrimsonR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Expression

Bacterial

Promoter

T5

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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