172,546 results

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RhoA sensor (LSS-SGFP2-mCherry)

Plasmid

#112948

Purpose

RhoA FRET biosensor

Depositor

Dorus Gadella

Insert

Rhoa sensor

Tags

LSS-SGFP2 and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pKOR1-mcs

Plasmid

#181755

Purpose

Allele replacement for Staphylococcus aureus

Depositor

Suzan Rooijakkers

Article

Stapels et al Proc Natl Acad Sci U S A. 2014 Sep 9;111

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pC-FLAG-ZCD2

Plasmid

#79274

Purpose

Mammalian expression of ZCD2 with a C-terminal FLAG tag

Depositor

Rita Shiang

Article

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

ZCD2 (CISD2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits
pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

Plasmid

#119723

Purpose

AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Thomas Oertner

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Inserts

CaMPARI2
CaMPARI2

Use

AAV

Tags

NES-his

Expression

Mammalian

Mutation

F391W, L398V

Promoter

hSyn1

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb713-HO

Plasmid

#141199

Purpose

Expresses mRhubarb713 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb713
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, W309R, Q310A compared to iRFP

Promoter

T7

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mito-meGFP

Plasmid

#172481

Purpose

meGFP targeted to the mitochondrial matrix with a human COX8 presequence

Depositor

Thomas Schwarz

Insert

Mito

Tags

meGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTBRII / CEP-Zeo / Hygro

Plasmid

#16622

Depositor

Bert Vogelstein

Article

Zhou et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Insert

TGF beta type II receptor (TGFBR2 Human)

Tags

HA

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
CDKN1A sgRNA

Plasmid

#138189

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDKN1A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

CDKN1A (CDKN1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-gp135

Plasmid

#222306

Purpose

Synchronize the trafficking of gp135/podocalyxin from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and gp135 fused to SBP-EGFP (PODXL Rabbit)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsLSm1_J

Plasmid

#146658

Purpose

Mammalian Expression of HsLSm1

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsLSm1 (LSM1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_BOP1:1-746

Plasmid

#211005

Purpose

Mammalian expression of full-length humanBOP1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

BOP1:M1-T746 (BOP1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-KNL1-rvsf_aaaa-dCas9

Plasmid

#248567

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

KNL1-rvsf_aaaa-dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

KNL1 RVSF58-61AAAA; Cas9 D10A, H840A

Promoter

CMV, TetO2

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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PB_iMYOD1_P2A_GFP_Puro

Plasmid

#168804

Purpose

PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

MYOD1 (MYOD1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pLX317-RBFOX1

Plasmid

#115442

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

RBFOX1 (RBFOX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pUCXMG

Plasmid

#204409

Purpose

Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

Depositor

David Ackerley

Article

Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

tac

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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Lv EF1a dCas9-HA 2A Blast

Plasmid

#102812

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

dCas9-HA

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A (dCas9)

Promoter

EF1a

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLz37_CB_operon_CsoS2_N1-N4

Plasmid

#140856

Purpose

Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Expression

Bacterial

Available Since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40

Plasmid

#100850

Purpose

AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1b

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-Ns3h-UGI

Plasmid

#221219

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-Ns3h-UGI

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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SPYtag-GFPnanobody

Plasmid

#214837

Purpose

This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-GFPnanobody

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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