We narrowed to 11,426 results for: Vars;

Showing: 6461 - 6480 of 11426 results

Results per page:

Sort results by:

pET15b MOSPD2 [315-445]

Plasmid

#186474

Purpose

Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [315-445]

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-SopB(172-554)

Plasmid

#183664

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 172-554

Promoter

CMV

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-SopB(211-554)

Plasmid

#183665

Purpose

N-terminally tagged Salmonella Typhimurium SopB truncation for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 211-554

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

Plasmid

#165083

Purpose

gRNA 2 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

Plasmid

#165085

Purpose

gRNA 1 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

Plasmid

#165086

Purpose

gRNA 2 of pair C for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

Plasmid

#169468

Purpose

Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

Plasmid

#169466

Purpose

Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSico_U6-PGC1a1 sgRNA

Plasmid

#165425

Purpose

Expression of gRNA against human PGC-1a variant 1

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

gRNA against PGC-1a variant 1 (PPARGC1A Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only
pCMV ShadowY-AURKA-mTurq2

Plasmid

#157770

Purpose

Expression of AuroraA kinase biosensor under CMV promoter

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

mTurquoise2 and shadowY

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v2-10 (-cyt)

Plasmid

#137825

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v2-10 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region - N213S on the CD44 re…

Promoter

PGK

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1 mitoAURKA Lys162Met

Plasmid

#157752

Purpose

Expression of kinase-dead AURKA K62M in mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP N1 AURKA 1-110

Plasmid

#157755

Purpose

Expression of AURKA Nter (aa1-110):GFP in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Mammalian

Mutation

The first 110 amino acids of AURKA were cloned

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP N1 AURKA 1-30

Plasmid

#157756

Purpose

Expression of AURKA Nter (aa1-30):GFP in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Mammalian

Mutation

The first 30 amino acids of AURKA were cloned

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1 AURKA deltaNter

Plasmid

#157749

Purpose

Expression of AURKA without Nter in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

the first 30AA of Aurora A are lacking

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCri-H6-TEV-GRAB

Plasmid

#128498

Purpose

Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)

Tags

His tag (6x)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCri-H6-TEV-GRAB-STREP

Plasmid

#128499

Purpose

Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)

Tags

His tag (6x) and Strep tag (2x)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP046

Plasmid

#107062

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting KU80 from Ogataea parapolymorpha

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting OpKU80 inO. parapolymorpha

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-Q03JI6

Plasmid

#103123

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

Q03JI6 (Cas9 coding gene from Campylobacter jejuni)

Use

CRISPR; Cloning vector

Tags

SV40NLS

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SPANXN4_WT

Plasmid

#81998

Purpose

Gateway Donor vector containing SPANXN4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPANXN4 (SPANXN4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge