We narrowed to 15,723 results for: LEA

1. pRevTRE MKL1 S449A/T450A/S454A

   Plasmid

   #19847

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Mutation

   changed Serine 449, Threonine 450 and Serine 454 …

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pL90-Nat-2xHO

   Plasmid

   #139069

   Purpose

   CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Insert

   Two guide RNAs targeting the HO endonuclease
   

   Expression

   Bacterial and Yeast

   Promoter

   TDH3

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pColdI-GST-mGTSF1l

   Plasmid

   #214817

   Purpose

   Bacterial expression of GST-tagged mouse GTSF1l

   Depositor

   Phillip Zamore

   Article

   Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

   Insert

   GTSF1l (Gtsf1l Mouse)
   

   Tags

   6x His and GST

   Expression

   Bacterial

   Promoter

   CspA

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT-MCS-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80058

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Tags

   3x HA

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_EE-NG-nCas9

   Plasmid

   #163542

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   EE-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

   Plasmid

   #75232

   Purpose

   CRISPR/Cas9 plasmid against human NFATc2

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against NFATc2 (NFATC2 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. DICER KO vector

   Plasmid

   #16656

   Depositor

   Bert Vogelstein

   Article

   Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

   Insert

   Dicer knock-out (DICER1 Human)
   

   Use

   Adeno-associated viral

   Expression

   Mammalian

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pClneoMyc MOB1 T12/35A

   Plasmid

   #47938

   Purpose

   expresses Myc tagged human MOB1 containing T12A and T35A mutations

   Depositor

   Yutaka Hata

   Article

   Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

   Insert

   MOB1A (MOB1A Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T12A and T35A

   Promoter

   CMV

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

   Plasmid

   #127865

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Mutation

   deleted the c-terminal 20 amino acids

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

    Plasmid

    #166413

    Purpose

    express hPOT1 ΔOB in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    Protection Of Telomeres 1 (POT1 Human)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    ΔOB deleted 125 a.a

    Promoter

    ef1-a

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2xNLS-NLP-cMyc LbaCas12a

    Plasmid

    #182123

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc LbaCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hy_pMT Flag-Hel25E K91N

    Plasmid

    #110121

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    K91N mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hy_pMT Flag-Hel25E D193E

    Plasmid

    #110120

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    D193E mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid

    #85820

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc Dam_f_GFPoNLS
    

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A.

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

    Plasmid

    #44597

    Depositor

    Susan Semple-Rowland

    Article

    Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

    Insert

    EF1a-GFP:2A:CHER(HA)
    

    Use

    Lentiviral

    Promoter

    human elongation factor -1a (pos 2528-3971)

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. ADGRL3-CTF-Flag

    Plasmid

    #201219

    Purpose

    pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

    Depositor

    Jonathan Javitch

    Article

    Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

    Insert

    ADGRL3 (Adgrl3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCHOP10(dLZ).pCDNA1

    Plasmid

    #21900

    Depositor

    David Ron

    Article

    Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

    Insert

    CHOP10 (Ddit3 Mouse)
    

    Expression

    Mammalian

    Mutation

    The coding region of the murine CHOP-10 cDNA was …

    Available Since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

    Plasmid

    #210504

    Purpose

    expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
    

    Expression

    Mammalian

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWay21-MKP-1 ASA

    Plasmid

    #13479

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1ASA (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Arginine 53 to Alanine, Changed Arginine…

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only