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MTK7_014

Plasmid

#123881

Purpose

Encodes the extended 3rd gen. 3' HIV LTR cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

3' HIV LTR (3

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSCL.028

Plasmid

#184965

Purpose

Test effect of extending -Eco1 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(extended), a1/a2 length: 27

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDYSCKO-ADE1

Plasmid

#120264

Purpose

pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

Depositor

Christian Landry

Article

Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

Insert

pDYSCKO cassette-ADE1 targeting guide

Use

CRISPR and Synthetic Biology

Promoter

SNR52

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-L1PA8CHmneo

Plasmid

#69608

Purpose

Expresses a neomycin tagged codon optimized L1PA8

Depositor

Astrid Roy-Engel

Article

Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

Insert

L1PA8

Tags

mneo retrotransposition cassette

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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PBDGTV

Plasmid

#100859

Purpose

A piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations.

Depositor

Yue Huang

Article

Liu et al Nucleic Acids Res. 2017 Sep 28. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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MTK234_032

Plasmid

#123995

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::HygroR-bGHpA

Plasmid

#62436

Purpose

MXS_chaining vector with PGK::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::tTA2-bGHpA

Plasmid

#62448

Purpose

MXS_chaining vector with PGK::tTA2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CnVA

Plasmid

#24587

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
MXS_CMV::HygroR-bGHpA

Plasmid

#62435

Purpose

MXS_chaining vector with CMV::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::CreERT2-bGHpA

Plasmid

#62444

Purpose

MXS_chaining vector with PGK::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pNVLTv2-pvdIJD-Ptrc-base

Plasmid

#169243

Purpose

Suicide vector for integrating expression cassette in P. putida (IPTG induction)

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

lacI

Expression

Bacterial

Promoter

lacIq

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCORE-Hp53

Plasmid

#72242

Purpose

Contains the CORE cassette with hyg and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
hyg

Expression

Bacterial

Mutation

V122A mutation

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b-mLin28A-cHIS

Plasmid

#137998

Purpose

Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

Depositor

Judy Lieberman

Article

O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

Insert

mouse Lin28A, CSD and ZnFs (residues 36–186)

Tags

C-terminal His6 tag

Expression

Bacterial

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWC

Plasmid

#112898

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_033

Plasmid

#123996

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA2 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_034

Plasmid

#123997

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA3 -hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTS2356_Tier3(PB)-PuroR

Plasmid

#169655

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::CreERT2-bGHpA

Plasmid

#62443

Purpose

MXS_chaining vector with CMV::CreERT2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pTV-Nanog Bgeo

Plasmid

#13837

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

IRES-Bgeo-pA cassette flanked by Nanog homologous regions (Nanog Mouse)

Use

Beta-gal

Expression

Mammalian

Mutation

None

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only